AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
5. TOXICITY ID: 7783-20-2
DATE: 18.04.2006
The chromosomal aberration rates in cells treated with
ammonium sulfate alone were not increased:
% ABERRANT METAPHASES (200 metaphases analyzed, including
achromatic lesions):
5.5, 6, 6, 3, 3 for controls without treatment, with 20 h
buffer treatment, with buffer plus ammonium sulfate
treatment (20 h), with 46 h buffer treatment, and with
buffer plus ammonium sulfate treatment (46h), respectively.
Test condition: Lymphocytes were isolated from whole blood in a Ficoll
gradient. 1x10e6 lymphocytes were incubated in 5 mL plastic
tubes in 2.5 mL McCoy`s 5A medium supplemented with 20%
fetal calf serum, 0.06 mL phytohemagglutinin M, 100 U/mL
penicillin and 0.1 mg/mL dihydrostreptomycin sulfate. The
cultures were incubated for 50 hrs, including a treatment
with colcemid (0.08 ug/mL) for 3 hrs. Treatment for 15 min
with Alu I, ammonium sulfate or Alu I shipping buffer was done
at culture times of 20 and 46 hrs, principally as described
previously (Obe and Winkel, 1985, Mut Res. 152, 25-29).
Shipping buffer consisted of: KPO4, 20 mM; KCl, 50 mM; EDTA,
0.1 mM; dithioerythritol, 10 mM; glycerin, 50% (v/v), pH 7.5.
Up to 7 independent experiments were performed with blood
from 5 different donors. Preparations were made following a
routine protocol and were stained with Giemsa stain.
The following aberrations were analysed: achromatic lesions,
chromatid breaks, isochromatid/chromosome breaks, chromatid
intrachanges, chromatid interchanges, triradials,
polycentric chromosomes, ring chromosomes and minutes.
YEAR OF STUDY: not reported.
Reliability: (2) valid with restrictions
limited documentation; only one dose level, no standard test
system for chromosomal aberrations
Flag:
Critical study for SIDS endpoint
07-NOV-2003 (140)
Type:
Cytogenetic assay
System of testing: CHO cells
Concentration:
0; 0.8; 1.6; 3.2 M (0; 106; 211; 423 mg/mL)
Cytotoxic Concentration: not reported
Metabolic activation: without
Result:
negative
Method:
GLP:
Test substance:
Result:
other: see Test Condition
no data
other TS: ammonium sulfate, not specified further
3.2M Ammonium sulfate did not induce chromosomal aberrations
in Chinese hamster ovary (CHO) cells. However, high
concentrations of ammonium sulfate, magnesium chloride,
calcium chloride or sodium chloride increased the frequency
of chromosome-type aberrations in CHO cells induced by the
restriction endonuclease Alu 1.
The results for cultures treated with Alu I, Alu I +
ammonium sulfate and control cultures were as follows:
Treatment / Percent aberrant metaphases / DIC*
Alu I / 40.7 / 34.3
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