AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
TEST CONDITION: Five 6-week old tadpoles were used in each
of three replicate aquaria, and the test was run for 10
days. Length and mean wet wet were measured at the end of
the test. The means and standard deviations of the
ammonium-nitrogen concentrations measured at the 5
experimental concentrations and in the control were 211.2
+/- 17.5, 118.0 +/- 9.8, 66.6 +/- 7.7, 37.0 +/- 4.6, 17.4
+/- 3.8 and 0 mg/L ammonium nitrogen, respectively. Ten day
LOEC values and NOEC values were determined with the
Dunnetts multiple comparison procedure.
Reliability: (1) valid without restriction
Flag:
Critical study for SIDS endpoint
22-JUL-2004 (67)
Species:
Expos. period:
Method:
GLP:
Test substance:
Remark:
Result:
Test condition:
136
other: Pseudacris regilla (Pacific treefrog)
10 day(s)
other: see Test Condition
no data
other TS: ammonium sulfate, 99.2% pure (Reagent Grade,
Mallinckrodt Baker Inc, Phillipsburg, NJ)
These tadpoles are from different egg masses than those used
for the 6 week-old test, (previous entry).
The NOEC for the tadpoles, which were 9 weeks old at the
start of the 10 day test, was 32.4 mg/L ammonium nitrogen.
The corresponding LOEC was 52.5 mg/L ammonium nitrogen.
TEST ORGANISMS: Eggs of Pseudacris regilla were collected
locally from non-agricultural areas and hatched in the EPA
laboratory facilities at Corvallis, Ore. Tadpoles were
subsequently reared in aquaria with a continuous flow of
temperature controlled fresh water whose temperature was
gradually increased from the collection temperature of ca.
10 0C to the test temperature of 20 + 1 deg C. Temperature
was continuously recorded. They were held at a photoperiod
of 14:10 light:dark. Newly hatched Pseudacris regilla were
fed pelleted rabit feed, with small amounts of newly hatched
brine shrimp and then frozen fish food.
TEST VESSEL: The test species were exposed to ammonium
sulfate in 18 10-L aquaria with a continuously-flowing water
diluter system that automatically delivered six
concentrations of chemical and controls to three aquaria per
concentration.
PHYSICAL/CHEMICAL PARAMETERS: Rearing and test water was
obtained from wells near the Willamette River at Corvallis,
Oregon. Dissolved oxygen, measured by electrode, was
maintained near saturation by the flowing water. pH (median
value 7.2) was measured by electrode. Total hardness (34 +/-
0 mg/L), alkalinity (34 +/- 0 mg/L), and conductivity (104.4
+/- 1.2 µS/cm) were measured by EPA methods 130.2, 310.1,
and 120.1, respectively. Background ammonium-N ranged from
0.005 - 0.010 mg/L in well water, and from 0.00 to 0.03 mg/L
in control tanks. Water samples for measurement of ammonium
sulfate were taken from each concentration (each aquarium in
the diluter) on two days, and analysed with a Hach DR/700
digital photometer. Quality assurance techniques were used
in the analysis. TEST CONDITION: Five 6-week old tadpoles
were used in each of three replicate aquaria, and the test
was run for 10 days. Length and mean wet wet were measured
UNEP PUBLICATIONS