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AMMONIUM SULFATE CAS N°: 7783-20-2

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OECD SIDS<br />

<strong>AMMONIUM</strong> <strong>SULFATE</strong><br />

4. ECOTOXICITY ID: <strong>7783</strong>-<strong>20</strong>-2<br />

DATE: 18.04.<strong>20</strong>06<br />

The 48 hour LC50 was 113,15 mg ammonium sulfate N L-1<br />

(converted from 15.5 mg NH4+/l); 5% confidence limit 13.75 -<br />

16.0 mg NH4+/l. The LC50 values, computed from the mortality<br />

rates, show that 50% of the organisms died within 49.8 hours<br />

at 15.0 mg NH4+/l, and that 50% died after 30 h at 75 mg<br />

NH4+/l.<br />

Test condition: TEST ORGANISMS: The green mussel Perna viridis was<br />

collectedfrom an unpolluted region at Someswara rocky shore<br />

(1<strong>20</strong>47'N;74051'E). All individuals were obtained during low<br />

tide from the sub-tidal belt. They were transported to the<br />

laboratory and kept unfed in large polythene trays, in<br />

aerated sea water for 24 hours before the beginning of the<br />

experiments. Young mussels in the size range <strong>20</strong> to 24 mm<br />

were used, and only members of the same population were<br />

examined in a single set of experiments.<br />

TEST VESSELS: Cylindrical glass troughs of 5 L capacity<br />

containing 4 l of sea water were used.<br />

TEST METHODOLOGY: Ten individuals were exposed to each<br />

concentration of NH4+, at 0, 5, 10, 15, <strong>20</strong>, 50, and 75 mg<br />

/L. Mortality tests were conducted over 96 hours. Death was<br />

defined as the inability to close the valves upon mechanical<br />

stimulus, and as valve gaping of 5mm. Dead individuals were<br />

removed from the media at 12 hour intervals.<br />

PHYSICAL/CHEMICAL PARAMETERS: The pH of the sea water varied<br />

between 8.0 and 8.3, and the temperature of the sea water<br />

varied between 28°C and 30°C during the experiments. In the<br />

presence of ammonium, the pH varied from pH 8.125 at 5 mg/L<br />

NH4+ to pH 7.85 at 75 mg/L NH4+.<br />

Reliability: (2) valid with restrictions<br />

Concentrations not measured. Limited documentation of<br />

results, and of the ammonium sulfate used in the experiment.<br />

Flag:<br />

Critical study for SIDS endpoint<br />

23-AUG-<strong>20</strong>05 (73)<br />

Type:<br />

flow through<br />

Species:<br />

Daphnia magna (Crustacea)<br />

Exposure period: 1<strong>20</strong> hour(s)<br />

Unit: mg/l Analytical monitoring: no data<br />

EC50: = 272<br />

EC100: = 500<br />

EC5 : = 110<br />

Method:<br />

GLP:<br />

Test substance:<br />

other: see Test Condition<br />

no data<br />

other TS: ammonium sulfate, analytical grade<br />

Test condition: Acute toxicity tests were performed with various materials<br />

used as fertilizer in Czechoslovakia.<br />

The tests were performed at pH 7.5 to 7.8 at 19-22 degree C<br />

with Daphnia magna Strauss. No detail is provided as to the<br />

experimental procedure (no. of test organisms, exposure<br />

etc.).<br />

Test concentration: 100, 10, 1 and 0.1 mg/l<br />

Reliability: (4) not assignable<br />

Abstract, original reference in foreign language<br />

26-JAN-<strong>20</strong>04 (68)<br />

Type:<br />

Species:<br />

104<br />

static<br />

Daphnia pulicaria (Crustacea)<br />

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