AMMONIUM SULFATE CAS N°: 7783-20-2

OECD SIDS
AMMONIUM SULFATE
4. ECOTOXICITY ID: 7783-20-2
DATE: 18.04.2006
Remark:
Result:
Test condition:
In this study, 27 commercial inorganic and organic chemicals
were tested simultaneously in seven aquatic species.
A LC50 value could not be determined because the proportion
killed at the highest dose level (100 mg/L) was less than
50%. No further details reported.
TEST ORGANISMS: Water flea (Daphnia magna) of first and
second larval instar. The test organisms were acclimated to
the control diluent water in the breeding/rearing tanks
(time period not reported). Food was withheld for the 24
hours preceding start of the test. Juveniles as uniform in
size as possible were collected from the colonies. Ten
juvenile organisms were exposed to the test chemical in each
treatment. Biological loading was kept below 0.5 gram wet
weight per liter of test solution.
TEST VESSEL: The assay was performed in seamless glass,
30.5-cm cuboidal, Pyrex chromatographic jars to which 20
liters of test solution was added. The test organisms were
segregated in welded stainless steel, 55-mesh wirecloth
baskets (5.5 cm in diameter x 7.5 cm in depth). Each basket
was suspended from a 1-rpm motor-driven mechanism that
raised and lowered the baskets in the water column. A
stainless steel band, slotted every 0.5 cm, facilitated the
positioning of the baskets so that the submerged volumes
changed from one-third to wo-thirds during each cycle.
One-half of the volume of the sumerged basket was exchanged
with the main tank volume every minute.
TEST SOLUTION: The test chemical was added directly to the
diluent water to give a nominal concentration of 100 mg/L.
The test chemical concentrations were not analyzed. Once the
test solutions were prepared, the starting temperature,
dissolved oxygen and pH values were determined for each
exposure concentration. When the starting pH of the test
soluton fell outside the extremes of 6.5 to 8.5, the pH was
adjusted to 7.0 by the addition of 10% (v/v) NaOH or 10%
(v/v) H2SO4.
DILUTION WATER: Water quality was routinely monitored to
characterize the diluent water and ensure its suitability
according to the standard method for the examination of
water and wastewater, 16th ed., American Public Health
Association, Washington DC pp. 689-832. The chemical
characteristics are reported in the publication and are in
accordance with standards required by current testing
guidelines. Activated carbon-filtered, dechlorinated and
tempered industrial service water from Lake Ontario was used
in all tests.
PHYSICAL/CHEMICAL PARAMETERS:
Determination of the temperature, dissolved oxygen and pH of
each test solution were made in conjunction with the daily
biological observation. The test temperature target was 20 +/-
1 °C. If the dissolved oxygen concentration in a test chamber
fell below 40% of the starting level in a test, the test was
repeated with 0.05 L/min glass-sparger aeration All tests were
conducted within the extremes of 6.5 to 8.5 pH units. The
photoperiod duration was 16 h of light. The air-water
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