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AMMONIUM SULFATE CAS N°: 7783-20-2

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OECD SIDS<br />

<strong>AMMONIUM</strong> <strong>SULFATE</strong><br />

4. ECOTOXICITY ID: <strong>7783</strong>-<strong>20</strong>-2<br />

DATE: 18.04.<strong>20</strong>06<br />

the logarithm transformation of the concentration and the<br />

angle transformation of the percent dead between the two<br />

doses that bracket 50% response.<br />

YEAR OF STUDY: not reported.<br />

Reliability: (2) valid with restrictions<br />

concentration not measured. Limited documentation of<br />

results.<br />

Flag:<br />

Critical study for SIDS endpoint<br />

26-JAN-<strong>20</strong>04 (59)<br />

Type:<br />

flow through<br />

Species:<br />

Gammarus fasciatus (Crustacea)<br />

Exposure period: 96 hour(s)<br />

Unit: mg/l Analytical monitoring: no<br />

LC50 : > 100<br />

Method:<br />

GLP:<br />

Test substance:<br />

other: see Test Conditions<br />

no<br />

other TS: ammonium sulphate, reagent-grade, no further details<br />

Remark:<br />

In this study, 27 commercial inorganic and organic chemicals<br />

were tested simultaneously in seven aquatic species.<br />

Result:<br />

A LC50 value could not be determined because the proportion<br />

killed at the highest dose level (100 mg/L) was less than<br />

50%. No further details reported.<br />

Test condition: TEST ORGANISMS: Sideswimmer (Gammarus fasciatus), 0.007 g.<br />

The test organisms were acclimated to the control diluent<br />

water in the breeding/rearing tanks (time period not<br />

reported). Food was withheld for the 24 hours preceding<br />

start of the test. Juveniles as uniform in size as possible<br />

were collected from the colonies. Ten juvenile organisms<br />

were exposed to the test chemical in each treatment.<br />

Biological loading was kept below 0.5 gram wet weight per<br />

liter of test solution.<br />

TEST VESSEL: The assay was performed in seamless glass,<br />

30.5-cm cuboidal, Pyrex chromatographic jars to which <strong>20</strong><br />

liters of test solution was added. The test organisms were<br />

segregated in welded stainless steel, 55-mesh wirecloth<br />

baskets (5.5 cm in diameter x 7.5 cm in depth). Each basket<br />

was suspended from a 1-rpm motor-driven mechanism that<br />

raised and lowered the baskets in the water column. A<br />

stainless steel band, slotted every 0.5 cm, facilitated the<br />

positioning of the baskets so that the submerged volumes<br />

changed from one-third to wo-thirds during each cycle.<br />

One-half of the volume of the sumerged basket was exchanged<br />

with the main tank volume every minute.<br />

TEST SOLUTION: The test chemical was added directly to the<br />

diluent water to give a nominal concentration of 100 mg/L.<br />

The test chemical concentrations were not analyzed. Once the<br />

test solutions were prepared, the starting temperature,<br />

dissolved oxygen and pH values were determined for each<br />

exposure concentration. When the starting pH of the test<br />

soluton fell outside the extremes of 6.5 to 8.5, the pH was<br />

adjusted to 7.0 by the addition of 10% (v/v) NaOH or 10%<br />

(v/v) H2SO4.<br />

UNEP PUBLICATIONS 99

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