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AMMONIUM SULFATE CAS N°: 7783-20-2

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OECD SIDS<br />

<strong>AMMONIUM</strong> <strong>SULFATE</strong><br />

4. ECOTOXICITY ID: <strong>7783</strong>-<strong>20</strong>-2<br />

DATE: 18.04.<strong>20</strong>06<br />

water and wastewater, 16th ed., American Public Health<br />

Association, Washington DC pp. 689-832. The chemical<br />

characteristics are reported in the publication and are in<br />

accordance with standards required by current testing<br />

guidelines. Activated carbon-filtered, dechlorinated and<br />

tempered industrial service water from Lake Ontario was used<br />

in all tests.<br />

PHYSICAL/CHEMICAL PARAMETERS:<br />

Determination of the temperature, dissolved oxygen and pH of<br />

each test solution were made in conjunction with the daily<br />

biological observation. The test temperature target was <strong>20</strong> +/-<br />

1 °C. If the dissolved oxygen concentration in a test chamber<br />

fell below 40% of the starting level in a test, the test was<br />

repeated with 0.05 L/min glass-sparger aeration All tests were<br />

conducted within the extremes of 6.5 to 8.5 pH units. The<br />

photoperiod duration was 16 h of light. The air-water<br />

interface of each tank received approximately 50 ft-c of<br />

cool-white fluorescent light.<br />

BIOLOGICAL PARAMETERS: biological observations were made<br />

daily. Survival, condition and behavioural information were<br />

recorded. Dead organism were removed when observed. If more<br />

than one-half of the population exposed in any treatment was<br />

found to be dead, additional aquaria containing lower<br />

concentrations of test soution were set up.<br />

EXPOSURE PERIOD: 96 hours.<br />

STATISTICAL METHOD: The 96-h LC50 value was derived using an<br />

interpolation method as described by Stephan (Stephan CE:<br />

Methods for calculating an LC50: Aquatic toxicology and<br />

hazard evaluation. In: FL Mayer and JL Hamelink, eds., STP<br />

634. American Society for Testing and Materials,<br />

Philadelphia, PA, pp. 65-84). The linear interpolation uses<br />

the logarithm transformation of the concentration and the<br />

angle transformation of the percent dead between the two<br />

doses that bracket 50% response.<br />

TEST CONCENTRATIONS:<br />

100, 10, 1 and 0.1 mg/l<br />

YEAR OF STUDY: not reported.<br />

Reliability: (2) valid with restrictions<br />

concentration not measured. Limited documentation of<br />

results.<br />

Flag:<br />

Critical study for SIDS endpoint<br />

26-JAN-<strong>20</strong>04 (59)<br />

Type:<br />

Species:<br />

static<br />

other: Agonus cataphractus (armed bullhead, hooknose, pogge),<br />

seawater fish<br />

Exposure period: 96 hour(s)<br />

Unit: mg/l Analytical monitoring: no<br />

Method:<br />

GLP:<br />

Test substance:<br />

other: see Test Conditions<br />

no<br />

other TS: ammonium sulfate, analytical grade<br />

UNEP PUBLICATIONS 85

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