Scientific Papers Series B Horticulture

Molecular differentiation showed a singlefragment of approximately 414bp wasamplified (Figure 2), according to Wang et al.,2003 which correspond to Xiphinema italiae.Figure 3. Xiphinema italiae Meyl, 1953: A, anteriorregion of female with lips region, odontostyle,odontophore, guiding ring; B, oesophageal bulb; C,posterior genital branch; D, anterior genital branch; E,head end; F, vaginal region; G, female tail; H, rectum.Scale bar: 20μm; 50μm.CONCLUSIONSXiphinema italiae was recorded on Prunuspersica for the first time in Romania.The monitoring of virus vector nematodesthrough soil samples, before set up the orchardsand vineyards, can assure healthy crop.Accurate identification of Xiphinema spp. isimportant in regard to their virus transmissioncapability.For a reliable diagnosis is necessary to combineidentification using morphometric charactersand molecular technics.ACKNOWLEDGEMENTSThis research was carried out with the supportof POSDRU/107/1.5/S/76888 project.REFERENCESAvgelis A.D., Tzortzakanis E.A., 1997. Occurrence anddistribution of Xiphinema species and Grapefanleaf nepovirus in vineyards of Greek island ofSamos. Nematologia Mediterranea, 25, p. 177-182.Barsi L., Lamberti F., 2003. Morphometrics of adults andjuvenile stages of three longidorid nematodes(Nematoda: Dorylaimida) from Vojvodina province,Northern Serbia. Nematol. Medit., 31, p. 65-85.Cohn E., Tanne E., Nitzany F.E., 1970.Xiphinemaitaliae, a new vector of grapevine fanleafevirus. Phytopatology, 60, p. 181-182.Gutiérrez-Gutiérrez C., Castillo P., Cantalapiedra-Navarrete C., Landa, B. B., Derycke, S., Palomares-Rius J. E., 2011. Genetic structure of Xiphinemapachtaicum and X. index populations based onmitochondrial DNA variation. Phytopathology 101,p. 1168-1175.Lamberti F., Agostinelli A., Radicci V., 1996.Longidorid nematodes from Northern Egypt.Nematol. Medit., 24, p. 307-339.Liškova M., Lamberti F., Sabova M., Valocka B.,Agostinelli A., 1993. First record of some specieslongidorid nematodes from Slovakia. NematologiaMediterranea, 21, p. 183-194.Martelli G.P., Cohn E., Dalmaso A., 1966. Aredescription of Xiphinema italiae Meyl, 1953 andits relationship to Xiphinema arenarium Luc etDalmaso, 1963 and Xiphinema conurum Siddiqi,1964. Nematologica, 12, p. 183-194.Nagy P., 1999. New faunistic record for Longidorusattenuatus Hooper, 1961 and Xiphinema italiaeMeyl, 1953 (Nematoda: Longidoridae) in Hungary.Növényvédelem, 35, p. 15.Peneva V., Choleva B., 1992. Nematodes of familyLongidoridae from forest nurseries in Bulgaria. II.The genus Xiphinema Cobb. 1913. Helmintology 32,p. 47-66.Romascu, 1971. Xiphinema americanum Cobb, 1913 siXiphinema italiae Meyl, 1953 (Nematoda:Dorylaimidae), noi daunatori ai vitei de vie, AnaleleI.C.P.P., vol. VII, p. 203-209.Seinhorst J.W., 1959. A rapid method for the transfer ofnematodes from fixative to anhydrous glycerin.-Nematologica4, p. 67-69.Teliz D., Landra B., Rapaport H.E, Camacho F.P., DiazJ. R. M., Castillo P., 2007. Plant parasitic nematodesinfecting grapevine in south Spain and susceptiblereaction to root-knot nematodes of rootstocksreported as moderately resistant. Plant. Disc., 91, p.1147-1157.Tzortzakanis E., Pateras D., Charoulis A., 2006.Occurrence of Xiphinema species in grapevine areasof Tyranvos with comments on the distribution of X.italiae in Greece. Helminthologia, 43, p. 186-187.Wang X., Bosselut N., Castagnone C., Voisin R., AbadP., Esmenjaud D., 2003. Multiplex Polymerase ChainReaction Identification of Single Individuals of theLongidorid Nematodes Xiphinema index, X.diversicaudatum, X. vuittenezi and X. italiae UsingSpecific Primers from Ribosomal Genes –Nematology, Vol. 93, No. 2, p. 160-166.161