Scientific Papers Series B Horticulture

could have a negative effect of environmentalcontamination with saprophytic germs (yeast,molds) and may be itself an undesiderablenutritional support for the development of thesegerms.Therefore, similarly I kept 3-5 anthers emptiedin the drops (v 3 ), to pursue these possiblenegative processes.After sowing the first laboratory tests weredone after an interval of 5 hours.Microscopic examination of samples taken inApril, was done by transmitted light and thephase contrast with objectives 10x, 20x, 40x.To maintain unaltered microscopicpreparations, both microspors extracted fromthe flowering buds (February-March) and themature pollen extracted from open flowers orbuds in the process of flowering (April) used toassess the viability and germinative capacity,were included after examination, in permanentpreparations in glycerin gelatin (Andrei et al.,2003).Figure 1. Pollen mother cells (PMC), Ob.20x, Oc.10xRESULTS AND DISCUSSIONSConcerning microsporogenesis and thedevelopment of young microspores, theflowering cherry buds taken in February it wasrevealed the normal development of anthers inappearance and size during organogenesis inthe three varieties studied.The flowering cherry buds of 8 March – werepointed out (highlighted) tetrades ofmicrospores that characterizes pre-swollen budstage (before flowering buds swelling). Theappearance being normally not observeddistartions of the microspores in tetrad ordisproportionate development betweenmicrospores. It was also observed theuniformity and normality of the cellular contentbetween tetrad components.The microscopic field were observed andpollen mother cells (PMC), due to nonsynchronizing of microsporogenesis process(Figure1, Figure 2 ).269Figure 2. Tetrade chain of microspores in cherry varietyBoambe Cotnari, Ob.20x, Oc.10xOn March 18 began to appear very youngmicrospores that still coexist with tetrades andthe flowering cherry buds of 21 March it wasobserved that anthers, microspores veryyoung contains, recently released from thetetrad. Exin contour was devoid of visibleornaments and was examined with objectives10x,20x,40x.Contour was approximately circular. Uniformappearance, almost spherical, is explained bythe lack of sporoderma stratification.The apertures not appear obvious just becauseexin is still thin and devoid (without)ornamentations. In some granules, aperturs aremarked by obvious folds.The flowering cherry buds of 25 March notedtjat microspores are large rand have amaximum size of approx. 23,5 . Change insizes and uneven appearance of microsporesare due to non synchronizing of maturationstage of microspores at different anthers ofsome bud.There are also differences from one variety toanother on the stage of maturation of