Scientific Papers Series B Horticulture

microspore to mature pollen, determination/analysisof pollen viability and germinationcapacity, and the relationship betweenvariations of media and dynamic pollen tubegermination, of three Romanian varieties ofcherry.These three varieties of cherry are available inour plantation of fruit trees and have not beeninvestigated (studied) and characterized fromthis point of view.MATERIALS AND METHODSIn 2011 there were evaluated microscopicallythree Romanian cherry cultivars with mediumand late ripening period: Severin, Daria, andBoambe de Cotnari. The age of the treesalternates between 7 and 9 years and belong tothe Baneasa SCDP collection. The samplesconsisted of flowering buds and open flowersthat were harvested as follows: flowering budswere collected in February-March and in Aprilthe flowers were collected in the first day ofanthezis and then the flowering buds.Flowering period lasted approximately 7 days.The flowering shoots were first fixed in Carnoysolution for several hours then preserved inethanol 70ºC (Andrei et al., 2003).Open flowers and the buds being opened(balloon stage) were analyzed immediatelyafter harvest (not being necessary orappropriate their setting and preserve).By dividing into sections (severing) theflowering buds that are being in progress(stage) before the swelling buds (pre-swelling)and swelling of the bud stage (March-April),we obtained the necessary data in the processof observing the early stages of themicrosporogenesis (tetrad with microspores,both very young and young microspores, thegradual appearance of the specific elements ofsporoderma, the apertures forming, etc.)The microscopical examination af samples inMarch has made on permanent preparations inglycerin gelatin, using optical microscopy IORtype ML4-M. It was used objectives 10x, 20xfor camera and 40x for microscopicexamination.For better observation (examination) of themorphological elements (features) mentioned,the preparations were stained with Carmin268Acetic Acid (ACA) or Methylene Blue vitaldye alcoholic solution (Andrei et al., 1972).They highlighted easier the differences betweenthe microspores with normal maturation of theimmatures, were identified viable microsporesby the non viables.At the pollen viability were estimated (V%)viability and germination capacity (G%) foreach variety separately. They used anthersextracted from several flowers forming ahomogeneons sample that represents faithfullythe biological potential of the pollen at thattime. Viability (V%) was expressed as apercentage in comparison of the viable pollenwith all pollen grains of microscopic fieldsexamined.To assess (estimate) the capacity ofgermination (G%), anthers were placed in eachsmall bottle as indicator (watch glass) and fewdrops of distilled water for hydration for pollenrelease.These unessential process and comparable withnatural hydration of pollen on the stigmasecretion (Xie B. et al., 2010).The pollen contained in each watch glass wasan sample mean for the cultivar examined.From the sample mean of each variety havebeen sowings of germination media twodifferent concentration of sucrose (15% and20%).The culture media that are used to the assess ofthe pollen germination are liquid media(distilled water) containing and 0,01% boricacid (H 3 BO 3 ).For each concentration of sucrose were seededthree versions (v 1 , v 2 , v 3 ) with which (by meansof them) was tasted the action of some flowerparts on (upon) germination.For the safety results were made in all threerepetitions (parallel sowing) as follows:- v 1 – the drop of liquid medium has beenseeded only with pollen;- v 2 – the drop of liquid medium has beenseeded with pollen together with pestle;- v 3 - the drop of liquid medium has beenseeded with pollen accompanied by emptyanthers.Pestle was introduced to try a simulation of theconditions in vivo referring to the stimulatingeffects that gineceu induced on the germinationrelease (on the stigma) and then on thedevelopment of the pollen tube. Pestle but