Scientific Papers Series B Horticulture

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The most abundant of the free amino acids inleaves were:- to the variety Galben de Odobeti: asparticacid (Asp), serine (Ser), threonine (Thr),glutamic acid (Glu), proline (Pro) andglycine (Gly), representing 74.7% of totalamino acids identified;- to the variety Zghihar de Hui: asparticacid (Asp), threonine (Thr), serine (Ser) andglutamic acid (Glu), representing 64.8% oftotal amino acids content;- to the variety Btut neagr: aspartic acid(Asp), glutamic acid (Glu), serine (Ser),threonine (Thr), and proline (Pro),representing 71.7% of the amino acidsidentified;- to the variety Negru moale: aspartic acid(Asp), glycine (Gly), serine (Ser), glutamicacid (Glu), threonine (Thr), and proline(Pro), representing 76.3% of total aminoacids content;- to the variety Negru vârtos: aspartic acid(Asp), glutamic acid (Glu), serine (Ser),threonine (Thr), arginine (Arg) and proline(Pro), represents 78.8% of total amino acidscontent;Statistical interpretation of data obtained ontotal amino acid content in leaves of vinevarieties belonging to Galben de Odobetisortogroup by analysis of variance/ANOVAtest shows that there is no statisticalsignificance between the five varieties, becausethe value of P is 0.938854, greater than thecritical value of P, 0.05.The ratio proline/arginine and the ratio ofpredominant amino acids (aspartic acid/serine)are considered parameters what remainconstant from year to year and differsignificantly from one variety/cultivar toanother and can be called descriptivebiochemical parameters. The ratio Pro/Arg hasvalues between 0.91 to the cultivar Negruvârtos and 1.68 for the cultivar Btut neagrand the ratio Asp / Ser ranges from 2.11 to thevariety Btut neagr and 2.73 to the varietyNegru vârtos (Table 2).Statistical interpretation of experimental dataobtained for the ratio Pro/Arg by analysis ofvariance/ANOVA test shows the existence of astatistical significance between the fivecultivars because P value is less than thecritical value of P, that is 0.05 (Table 3).Table 2. The values of biochemical descriptors for grapevine varieties to Galben de Odobeti sortogroupThe variety/Biochemical descriptorsGalben de Odobeti Zghihar de Hui Btut neagr Negru moale Negru vârtosPro/Arg ratio 1,29 1,15 1,68 1,28 0,91Asp/Ser ratio 2,33 2,65 2,11 2,65 2,73Table 3. The significance of differences to the ratio proline / arginine in the leaves of vine varieties to Galben deOdobeti sortogroupThe vine variety Galben de Odobeti Zghihar de Hui Btut neagr Negru moale Negru vârtosGalben de Odobeti 0 Zghihar de Hui 0.06542 0 Btut neagr 0.00051 0.00064 0 Negru moale 0.94536 0.06777 0.00093 0 Negru vrtos 0.01346 0.06722 0.00096 0.01368 0Statistical the ratio proline/arginine geneticallydifferentiates the variety Btut neagr theother varieties of Galben de Odobetisortogroup, with a confidence interval of 95%.A significant difference exists between thevariety Negru vârtos and the varieties Galbende Odobeti and Negru moale. Varianceanalisys/ANOVA test for the ratio predominantamino acids (aspartic acid/serine), statisticallydifferentiates the variety Btut neagr by theother varieties except the variety Galben deOdobeti with a confidence interval of 95%,while this difference is not significant for thevariety Galben de Odobeti (Table 4).167
Table 4. The significance of differences in the ratio aspartic acid / serine of leaf vine varieties of Galben de OdobetisortogroupThe vine variety Galben de Odobeti Zghihar de Hui Btut neagr Negru moale Negru vârtosGalben de Odobeti 0 Zghihar de Hui 0.11292 0 Btut neagr 0.23710 0.00732 0 Negru moale 0.08869 0.93531 0.00357 0 Negru vârtos 0.06510 0.44839 0.00510 0.39683 0CONCLUSIONSThe total free amino acids content in leavesvaries between 18.76 g/mg fresh leaf tissue tothe variety Galben de Odobeti and 14.33g/mg to the variety Negru moale, withintermediate values for the variety Negruvârtos (17.74 g/mg), (cv) Btut neagr(15.20 g/mg) and (cv) Zghihar de Hui(14.54 g/mg).The most abundant of the free amino acids inleaves of native varieties to Galben deOdobeti sortogroup are: Asp. Ser. Thr. Glu.Pro. Arg and Gly. Aspartic acid is found inlarge amounts compared with other aminoacids predominant.The ratio proline/arginine has differentiatedstatistically the variety Btut neagr by theother varieties of Galben de Odobetisortogroup with a confidence interval of 95%.Significant difference exists between thevarieties Galben de Odobeti and Negruvârtos and between the varieties Negru moaleand Negru vârtos.The ratio between predominant amino acids(aspartic acid/serine) has differentiatedgenetically the variety Btut neagr by theother varieties (except the variety Galben deOdobeti) with a confidence interval of 95%.There is significant difference between thevarieties Galben de Odobeti and Negruvârtos and between the varieties Negru moaleand Negru vârtos.The amino acid profile of leaves can be used asdiscriminant analysis method of vine varietiesto Galben de Odobeti sortogroup. with theother modern methods of investigation(isoenzyme analysis and DNA analysis).ACKNOWLEDGEMENTSThe authors are grateful Mr. Ass. Prof.Gheorge Stoian, Ph.D., in the Department ofBiochemistry and Molecular Biology,University of Bucharest, Faculty of Biology,for their help in working with TLC.REFERENCESBieleski R.L.. Turner N.A., 1966. Separation andestimation of amino acids in crude plant extracts bythin-layer electrophoresis andchromatography. Anal. Biochem., p. 278–293.Hernández-Orte P., Guitart Ana, Cacho J., 1999.Changes in the Concentration of Amino AcidsDuring the Ripening of Vitis vinifera TempranilloVariety from the Denomination d'Origine Somontano(Spain). Am. J. Enol. Vitic. 50:2, p. 144-154.Huang Z., Ough C. S., 1991. Amino Acid Profiles ofCommercial Grape Juices and Wines. Am. J. Enol.Vitic. 42:3, p. 261-267.Kliewer W.M., 1967. Annual cyclic changes in theconcentration of free aminoacids in grapevine. Am. J.Enol. Vitic. 18., p. 126–137.Shiraischi Mikio, 1996. Proposed BiochemicalDescriptors for Amino Acids to Evaluate GrapeGermoplasm, J. Japan. Soc. Hort. Sci. 65(2), p. 283-289.ârdea C., 2007. Chimia si analiza vinului, Ed. “IonIonescu de la Brad, p. 754-770.168
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SCIENTIFIC PAPERSSERIES B. HORTICUL
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SCIENTIFIC COMMITTEE Bekir Erol AK
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Fructification - Florin Constantin
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Phenological Studies on Some Variet
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Scientific Papers. Series B, Hortic
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Table 2. The organoleptic appreciat
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processes become even slower and th
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- usingselectedbacteriafromm Leucon
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To organoleptic analysis of the win
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works were pickling the cucumbers i
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days after removal of weeds by burn
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it appears that the culture has gro
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Maintainance of the genetic structu
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Table 3. Interaction variety of dwa
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Experimental scheme is situated in
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improvement especially laboratory s
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Table 1 shows the type 2x3x2 trifac
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Neamtu G., Gheorghe Campeanu, Carme
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Experience consisted of the followi
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Table 7. Total production on plantV
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vineyard, several methods were used
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Table 4. Structure and number and r
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gets serious role through the ferme
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Table 3. Element content of the ber
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Figure 4. The length of internodes
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The process of fruit forming was de
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Compomist F1 hybrid reaches value o
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Figure 2. Experimental results repr
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and total number of tubers per plan
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and quality of organic potatoes for
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Petri dishes, the pathogenic fungi
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Variant 4R 2 11.0 4.0 15.0 7.0 22.0
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Analyzing the dynamics of the total
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Table 2. Indicative data on volumes
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Table 3. The Influence of the level
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Table 8. The Influence of irrigatio
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Figure 1. The aspect of apples from
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Conclusions on changes in chemicalc
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RESULTS AND DISCUSSIONSRaspberry is
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CONCLUSIONSAs a result of scientifi
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observations were made on phenologi
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Figure 1. Flowering period and blac
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Page 118 and 119: content, A-acidity content) to the
Page 120 and 121: sugar accumulated in grapes and 1,0
Page 122 and 123: Scientific Papers. Series B, Hortic
Page 124 and 125: Figure 2. DryerFigure 3. Programmer
Page 126 and 127: Appearance of the apricots after 5
Page 130 and 131: where: P F represent the coordinate
Page 134 and 135: Table 2. The production obtained at
Page 136 and 137: As shown in figures 5 and 6 the vit
Page 140 and 141: At two weeks from planting it was a
Page 142 and 143: If we take into account the both fa
Page 146 and 147: F1 - + infectedF2 - + infectedF3 +
Page 148: HORTICULTURALBIODIVERSITY ANDGENETI
Page 151 and 152: RESULTS AND DISCUSSIONSFruit charac
Page 156 and 157: The chorology maps of Artemisia alb
Page 158 and 159: Figure 5. Chorology of Artemisia le
Page 162: Molecular differentiation showed a
Page 165 and 166: The characterization of the grapevi
Page 167: Figure 4. Amino acid profile of the
Page 171 and 172: The studied phenophases (table 1),
Page 173 and 174: REFERENCESCapusan Janina, 2013. Rez
Page 175 and 176: Neoaliturus fenestratus Herrich-Sch
Page 177 and 178: occurrence in western part of Roman
Page 179 and 180: Pop 3 = Population 3 obtained by cr
Page 181 and 182: well adapted in our country, and th
Page 186 and 187: Variations in acidity of the variet
Page 188: grape. Analele Universitatii din Cr
Page 191 and 192: Flow cytometry has proved to be an
Page 193 and 194: cell nuclei, using chromosome count
Page 195 and 196: Figure 1. Prekos F1Figure 4. Prekos
Page 197 and 198: Interpretation of the results conce
Page 199 and 200: Photo 1- Redhaven (Control)The Roma
Page 201 and 202: Dry matter (determinate refractomet
Page 203 and 204: was evaluated by using a large-size
Page 208 and 209: During the growing season have been
Page 212 and 213: Likewise, vitamin C content of ‘G
Page 216 and 217: Figure 2. Air temperature (°C) in
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REFERENCESAnconelli S., Antolini G.
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Taking into the account these and t
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postharvest decays in fruits and ve
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manure semifermentated. In vegetati
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Figure 1. Prima Cl. 1022 varietyThe
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temperature required for a differen
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MATERIALS AND METHODSTo accomplish
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climate (IH4), becomes for this yea
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quantities of sugar accumulated in
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ibosomal to investigate 13 species
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Biogeography of Genus MangiferaTwo
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productionand uses. Center for trop
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analysis of variance to express the
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highlighted by a correlation coeffi
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without heat and in open field. Cro
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ut the fruit had a reduced number o
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Genetic autochthonous heritage wasi
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Table 1. The main characteristics o
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Data were subjected to statistical
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Also, the dry biomass and root leng
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“Clapp’s Favorite” in 2011 wh
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2012 the order was “Conference”
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microspore to mature pollen, determ
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microspores at certain date. Micros
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viin utilizate ca genitori potentia
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The objective of this paper, is to
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Table 1. Relationship between germi
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Figure 2. Tassels lengthIn case of
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ORNAMENTAL PLANTS,DESIGN ANDLANDSCA
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of Chisinau, also the old parks. Fo
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y cambium on the very center - meth
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flower is fully colored. Postharves
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Results from table 8 shows that Mar
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Pinus sylvestris ‘Gold Coin’, R
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Figure 4. Excelsa (H 18/1)Descripti
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Figure 1. Plants in cellular trays
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espectively 69%, followed by hybrid
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observations made on the plants of
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The values of pH diminished in all
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Figure 5. Content of total soluble
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Figure 1. Plan of Bran domain (Ion
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Carol the second, her brother, on h
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Figure 14. Tea house in the natural
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Cults, and in conformity with the c
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consult the scientific novelties an
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,,The restoration is the methodolog
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MATERIALS AND METHODSThe chorology
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Merce, 2011; Zamfirescu, 2010) (IAS
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experiment because it shows and ill
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among all the administrations, exce
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solution known as AKN. Content: pot
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As shown in the figure, Chrysal is
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MotivationsOfferprospectingCustomer
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conclude the presented case, with k
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Also, comparative studies, concerni
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done before. Also the speed of the
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as Marcellus theatre or Trajan foru
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ian regime, by huge contradictions
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Pyongyang, the hidden cityThe capit
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“ordinary” buildings as the gen
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- The Writer’s Rotunda“The cons
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Figure 4. Vegetation planRegarding
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Figure 9. BenchesVasesThere are 4 k
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REFERENCESPanoiu A., 2011, Evolutia
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RESULTS AND DISCUSSIONSResults of t
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Figure 5. Average growth rate of
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In 2012 there were higher onset tem
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fact that root injuries is the majo
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We recommend that the species and c
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and parameters of quality. The phys
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AESTHETICALLEVEL OFAPPROACHLEVEL OF
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REFERENCESArchibugi F., 1997. The E
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Figure 1. Optimized version of comp
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CONCLUSIONSThe optimized version (F
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etween 182-212 g/l, the lowest valu
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CONCLUSIONSThis study showed that 2
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RESULTS AND DISCUSSIONSFor this pur
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according to quality class.The calc
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third party countries who wish to b
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Stakeholder comments concerned nota
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Figure 2. The „Eco-leaf” logo o
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identified to facilitate its tracea
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- Analysis reports;This document ma
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Table 1. Sampling procedures applic
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Table 2. Example of sampling proced
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should be 5 kg.” According to thi
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One of the first and simplest condi
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of RIN transcription factor in ethy
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oriented towards the centre of the
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Figure 5. Macroscopic view of the c
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Related to the process of the evolu
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efore it is printed using the main
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Imposition of the pot is done every
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Figure 8. Test of Strength Vetiver
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Table 3 defines LSD test for genoty
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