Scientific Papers Series B Horticulture

microspores at certain date. Microspores aresignificantly different from those of 21 Marchas they start to differentiate at sporodermaslevel weaks ornamentations and we observe theapertures shape. However it also notes thecytoplasmic granulation at the granules withstill thin sporoderma .Concerning mature pollen: as the floweringcherry buds of 6 April, sporoderma has aspecific appearance for mature pollen, weproceeded of ots application to test theviability. Were revealed the microsporesappearance with a normal development forapprox. 70% in deep red and yellowmicrospores containing reduced or absentcell and pollen with methyl blue stainingvital dyestuff alcoholic solution, to highlightthe presence of oil droplets that alwaysaccompany young pollen maturation (Figure 3and Figure 4).Viability and germinative capacity of the threeRomanian varieties of cherry have maximumvalue for version 2 (v 2 ) with 20%sucrose. Boambe de Cotnari cultivar hadviability (V%) 80% and maximumgermination (G%) of 48%. Severinvariety had viability (V%) 75% and maximumgermination (G%) of 25% and Daria varietyhad viability (V%) 60% and the maximumgermination (G%) of 18%.As currently practiced were considered asbeing germinated the grains that has pollentube lenght at least equal twice the diameter ofpollen.Figure 4. Staining Pollen with methylene blue vitaldyestuff alcoholic solution, to highlight the presence ofoil droplets that always accompany young pollenmaturation in Daria variety, Ob.20x, Oc.10xThe results of 3 repetitions for eachexperiment, were expressed as a percentagebased on the corresponding arithmetic mean(Figure 5).The pollen tube length (PTL) / varietycorrelated with average sucrose % in variantsv 1 , v 2 , v 3 are thus: pollen tube germinated atcherry varieties in the Romanian culture media,varies in length from approx. 30 to about400. (Figure 6).To determine if a relationship exists betweenpollen tubes development and the medium thepollen germinated it was formed the graphicwhich were placed at intervals in order oflength () all „PTL max” and the correspondingexperiments (Figure 7).Figure 3. Red color, intense reaction to viable maturepollen during the test for viability, in cherry Severinvariety, Ob.10x, Oc.10x.Figure 5. Dynamic pollen (V% and G%) for 3 varietiesof cherry in variants (v1,v2.v3) with 20% sucrose.270