Scientific Papers Series B Horticulture

espectively 69%, followed by hybrid MiragePastel Pink with a germination rate of 64%.The lowest percentage of germination wasobtained in hybrid Flamenco White of only62%.The data obtained are in accordance with theliterature, which mentions a low germination inthis species, both because of the very small sizeof seeds and germination special conditions,namely the presence of light (Cantor, 2009).Results for rooting cuttingsData on percentage of rooting cuttings fromthree hybrids analyzed are presented in Table 2.Table 2. Rooting percentage in the three LisianthushybridsVariant 1Number ofrootedcuttingsusedNumber ofrooted cuttingsPercentage ofrooting(%)S1E 30 0 0S2 E 30 5 16S3E 30 3 10S1F 30 0 0S2F 30 4 13S3F 30 3 10S1M 30 0 0S2M 30 8 26S3M 30 4 13Total270 27 10number1Substrate 1Hybrids;S1-river sand; E-Echo Lavender;S2-peat + sand, mixture 1:1; F-Flamenco White;S3-peat + sand + garden soil, mixture 1:1:1; M-Mirage Pastel PinkFrom the table it can be seen that the highestpercentage of rooting was obtained in hybridMirage Pink Pastel on sand and peat substratemixed 1:1 with 26%, followed by hybrid EchoLavender on the same substrate, 16%. A smallpercentage of rooting was recorded and thesubstrate composed of soil-peat-sand in a ratioof 1:1:1. Pastel Pink Mirage hybrid resulted ina 13% and other hybrids with a percentage of10%. If the substrate consists of river sand hasno rooted cuttings.CONCLUSIONSBased on the experiences made the followingconclusions are:Data obtained shows that the seeds size andecological conditions influenced negatively thegermination percentage, registering an averageof only 65% for the analyzed hybrids.The experiments conducted shows that speciesLisianthus russelianus Hook. is quiterecalcitrant to conventional vegetativepropagation, by cuttings, regardless of thesubstrate used.The small percentage of rooting, probably dueto the fact that greenhouse facility was notequipped with 'mist system' that does notensure a high air humidity nor stimulators wereused rootedness.We recommend the promoting culture in ourcountry, of the species Lisianthus russelianusHook., by enriching assortment with newcultivars.Biological material obtained by applyingconventional propagation methods lead toobtaining parent plants which can be used as abasis for in vitro multiplication that willproduce an unlimited number of plants in arelatively short time.REFERENCESAli A., Afrasiab H., Naz S., Rauf M., Iqhbal J., 2008. Anefficient protocol for in vitro propagation of carnation(Dianthus caryophyllus L.). Pak. J. Bot. 40: 111-121.Ardelean M., Sestras R., Cordea M., 2007. Tehnicaexperimentala horticola, Ed. AcademicPres, Cluj-Napoca.Bala Maria, 2010. Floricultura speciala, Ed. Timpolis,Timisoara, p. 320.Cantor Maria, 2009, Floricultura generala, Ed. Todesco,Cluj-Napoca, p. 64-65.Harbaugh B.K., 2006, Lisianthus, Eustomagrandiflorum. In: Anderson NO (Ed), FlowerBreeding and Genetics, Springer, Netherlands, pp645–663.Kanwar J.K., Kumar S., 2009. Influence of growthregulators and explants on shoot regeneration incarnation. Hort. Sci. 36: 140-146.Roh M.S., Lawson R.H., 1988. Tissue culture in theimprovement of Eustoma. HortSci 23, p. 658.Roh M.S., Halevy A.H., Wilkins H.F., 1989. Eustomagrandiflorum. In: Halevy AH (Ed), Handbook ofFlowering, CRC Press, Boca Raton, FL, pp 322–327.Toma F., 2009. Floricultura si arta florala, Vol. 1, Ed.Invel Multimedia Bucuresti.307