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Scientific Papers Series B Horticulture

Scientific Papers Series B Horticulture

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Related to the process of the evolution of theinhibition percent values we observed that thisis correlated with growth rate of thesaprophytic fungi.The fact the fungus Trichothecium roseumshowed a higher inhibition than the fungus E.nigrum that had a faster growth, is probablydue to the capacity of the species T. roseum togrow over and hyperparasitize the hyphae ofthe pathogens, while colony of the fungusE. nigrum was slowly surrounding the colonyof Mycosphaerella grossulariae.Table 9. Inhibition level exercited by saprophytic fungion M. grossulariae in experimentally variantsVariantM. grossulariae/ T.roseumM. grossulariae/ G.roseumM. grossulariae/ E.nigrumM. grossulariae/ T.virideControl (M.grossulariae)after 7daysInhibition levelAfter 10 after 30days daysafter 45days3 5 8 81 3 7 83 4 8 84 5 8 80 0 0 0Figure 10. Evolution of inhibition level (0-10) inexperimentally variantsCONCLUSIONSThe best vegetative growth of Mycosphaerellagrossulariae(Auers.) Lind. CBS 235.37mycelia was registered on PDA medium, thistype of medium being selected for growth fungiestimation used in this study and for inhibitioneffect of saprophytic fungi evaluation.Among the 5 species incubated on PDAmedium, the fast growing was observed in thecase of the strain of Trichoderma viride,closely followed by the isolates ofEpicoccumnigrum and Trichothecium roseum.Among saprophytic fungi the length growthwas observed in the case of Gliocladiumroseumisolate.The strain of Mycosphaerellagrossulariae hadthe lowest rate of growing, reaching a mediumvalue of 7.3 mm of colony diameter after 6days in comparison with the saprophytic fungiTrichoderma viride which totally covered thePetri dish surface.All fungi tested related to their inhibitorycapacity were influenced significantly thepathogenic fungus growth, but the strongerinhibitory effect was observed in the case of thestrain of Trichoderma viride, followed by thoseof Trichothecium roseum and Epicoccumnigrum.The lowest inhibitory effect in thefirst 10 days after inoculation was observed inthe case of Gliocladiumroseum strain, but after45 days the inhibition value (I%) was closely tothe other variants.The inhibitory effect was direct proportionalwith the growth rate of both fungi: pathogenicone and the antagonistic with the exception ofTrichothecium roseum and Epicoccumnigrum.Although Trichothecium roseum had a morelength growth rate in comparison withEpicoccumnigrum it expresses a strongerinhibitory effect.ACKNOWLEDGEMENTSThe present study was performed accordingwith the objectives of the project PN-II-62-079/2008 SIMAECO (CNMP-UEFISCDI).435

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