Scientific Papers Series B Horticulture

Eustoma randiflorum is commonly propagatedby seed or cutting. Sexual propagation used toobtain seedlings seeds brought from abroad(Netherlands, USA, France, etc). From a gramof seeds can be obtained about 8000 plants(Bala, 2010).Generally, sowing the plants of Lisianthus is inautumn or January-February. Industrial culture,sowing can be done in half, and according tothis period, the number of days to floweringmay vary.Propagation by cuttings is less practiced.Cuttings are cut to a length of 10 to 15 cm(which have three pairs of leaves) and placedon the substrate of peat and perlite, sand orperlite simple. Rooting takes place for 40-50days. The disadvantage of this method is thatthe percentage of shoots of the plant is weaker,we get a few plants and can easily transmitdiseases and pests (Toma, 2009).MATERIALS AND METHODSFor the new species introduced are requiredefficient multiplication techniques to beestablished. The study was about conventionalpropagation methods, by seeds. The biologicalmaterial used in the experience comprised inseeds from Sakata Company, represented bythree F 1 hybrids of Lisianthus russelianus:„Echo Lavender”, „Flamenco White” and„Mirage Pastel Pink”. These were used toobtain plants to start the in vitro and in vivoexperiments in order to establish thegermination and the rooting rates. Seeds weretreated thiuram pelleted. The biologicalmaterial presents the following characters:,,Echo Lavender”. It is one of the mostpopular cultivars with early flowering. Plantsare vigorous in spring, while in summerdroughts become more fragile. The leaves are5-8 cm long, elliptical, slightly sharp andsucculent; they look dull and bluish-greencolor, with 3-5 ribs clearly visible. Summerflower buds open in cones than 5 cm indiameter, showing many shades of purple.,,Flamenco White”. The flowers are simple, issuitable for cultivation in summer whentemperatures are high and days are long. Stemsare vigorous and flowers on top of them arewhite. Leaves are skin, colored bluish green,located opposite the stem. Stem height is 50cm. Blooms two weeks earlier than hybridMirage Pastel Pink.,,Mirage Pastel Pink”. Flowers are simple,ideal for summer flowering. Has smaller petals,and are resistant to transport. The flowers arepink, with the little white. The leaves are greenand vigorous stems are about 40-50 cm inlength. It is resistant to high temperatures andbotrytis.Experience was made in greenhouses ofUSAMV Cluj-Napoca and the greenhouses ofthe Botanical Garden Alexandru Borza Cluj-Napoca.Propagation by seedsSowing was done on 04.03.2011, in smallpockets trays using the neutralized peatsubstrate. The seeds were sown on the surfacebecause they are photosensitive (germinate inthe presence of light). Greenhouse temperaturewas 20-22°C during germination, and thendecreased to 18-20°C. Relative humidity was70-75% and 80-85% of the substrate,maintaining this level through daily watering.Germination occurred at 21 days, after whichobservations were made on the percentage ofgermination. During the period of germinationwere assured maintenance, or weeding,watering and ventilation greenhouse. Lisianthusfrom each hybrid were sown one hundredseeds.After about six weeks on 4/24/2011 plantsproduced two little leaves true. Four days later,on 05.28.2011 was carried out firsttransplantation in alveolar larger trays, then on6/10/2011 moved in pots 7x7cm (Figure 1).304