Scientific Papers Series B Horticulture

Table 3. The vegetative growth of the fungusMycosphaerella grossulariae (Auers.) Lind.CBS 235.37and the saprophytic fungi Trichoderma viride,Epicoccum nigrum, Gliocladium roseum andTrichothecium roseum grown on PDA mediumColony diameter (mm)Variantafter 3 days after 6 daysTrichoderma viride 61.6 68.0Epicoccumnigrum 31.0 63.0Gliocladiumroseum 13.6 31.3Trichothecium roseum 26.3 55.0M. grossulariae 5.0 7.3Figure 2. Vegetative growth of the colonies of the fungusMycosphaerella grossulariae (Auers.) Lind.CBS 235.37on different culture mediaAmong the five fungi grown on PDA medium,pathogenic fungi showed a significantly lowergrowth rate than saprophytic fungus Trichodermaviride. The other saprophytic fungishowed also significantly higher growth rates,but lower than Trichoderma viride (Figure 3,Table 3).On the whole period of experimentation, in thecontrol variant, fungusMycosphaerellagrossulariae had a constantlygrowing rate and reaching a medium value of5.91 mm after 7 days after inoculation of thesaprophytic fungus in variant (Figure 1a), 7.3mm after 10 days (Figure 1b), 15.8 mm after 30days, 17.9 mm after 40 days and finally 18.7mm after 45 days (Figure 1c). The shape ofcolonies was circular. The colour was whitewith the reverse of light brown (Figure 4).Figure 4. Macroscopic view of the colony ofMycosphaerella grossulariae (Auers.) Lind.CBS 235.37on PDA medium after 7 days (a), after 10 days (b) andafter 45 days (c ) from pathogen inoculation in variantsFigure 3. Diameter of the colonies of the strains of thefungi Trichoderma viride (Td), Epicoccum nigrum(E.n.),Gliocladium roseum (G.r.), Trichothecium roseum (T.r.)and the strain of the pathogenic fungus Mycosphaerellagrossulariae (Auers.) Lind.CBS 235.37 (M.g.) on PDAmedium432The strain of Trichothecium roseum had a fastgrowth to the pathogen strain of Mycosphaerellagrossulariae.After 6 days from itsinoculation, the mycelia of Trichotheciumroseum began to cover all the area around thepathogen and blocked its expansion by theinhibitory effect under the internal radius whichpointed the value of 3.95 mm read on thereverse side. After 7 days the Trichotheciumroseum pink mycelium had developed aroundthe white Mycosphaerellagrossulariae colony(Figure 5a). The division line had his concavityto pathogen fungi oriented who had a low rateof growing. The external radius continuoslygrowing against internal radius, the valueregistered being of 4.4 mm. After 10 days themycelium of T. roseum had completely coveredthe pathogen colony which being totallyinhibited (Figure 5b, Figure5c).