Scientific Papers Series B Horticulture

The objective of this paper, is to evaluate thepotential fertility pollen, in laboratoryexperimental conditions.These apricot hybrids experienced in thisresearch, are existing in ours fruit treeplantation and have not been examined / testedand characterized and this point of view. thepollen germination (in vitro), in liquid medium(deprived of agar-agar), because we consideredit may be relatively comparable, withintercellular fluid composition of stylar tissue,that pollen tube develops, and allow betterdispersion of pollen and thus favors in liquidmedium, the development of pollen tubes.MATERIALS AND METHODSNicusor cultivar. Age of the trees is 8, withmedium and late ripening period. In April (onthe first day of anthesis in 04.04.2012), therewere taken from open flowers and floweringbuds for determing the viability and thecapacity of pollen germination.To asses the viability and to represent as realpossible the biological potential of the pollen atthat time, to each sample and analysis partly,were used fresh anthers that were extractedfrom the stem filaments of the flowers orcurrent flowering buds.directly on microscope slide glass, there wereapplied (according to the Method Andrei andParaschivoiu, 2003), directly on the fresh andmature pollen, e few drops reagent 2,3,5-Trifeniltetrazol Clorid, for coloring reaction(Andrei and Paraschivoiu, 2003).The pollen viability was evaluated under amicroscope in transmitted light, by examiningof the morphological and physiologicalcharacteristics of the pollen cell.The method is recommending the counting thecolored grains only as a result of the deep redreaction (Andrei and Paraschivoiu, 2003).The viability was expressed in per cent (V%)confronted by the total grains counted in thefield by reporting of the viable pollen to totalgrains of microscopic fields examined.For creating the media culture in vitro todetermine / assess germination capacity (G%)of the mature pollen, was hydrated (previously)the pollen, released from anthers fresh , onseveral slides glass.This essential process is comparable withnatural hydration of pollen on the stigmasecretion (Xie B. et al., 2010).Then separately, were made sowing ongermination media in 2 different and separatedconcentration of sucrose (15% and 20%) inwhich added 0,01% boric acid (H 3 BO 3 ).We believe that, the pollen germinationadvantage on the liquid medium directly onslide glass, allows at better microscopicexamination through transparency and thetransmited light unlike the germination on solidmedium in Petri dishes, which cannot beexamined only in direct light at o “powerenlargement“ of max. 200x for stereomagnifier.Also the pollen germination testing, was donein 3 different germination the tasting in 3germination variants (v1,v2,v3) by introducingof some floral parts (ginaeceum / pistil and theremaining emptied anthers), in germinationmedium to emphasize their influence / actionon pollen germination (Iordache et.al., 2010).So each microscopic slide was a test variant(V1,V2,V3) thus as follows:- Variant V1- liquid drops was seeded onlywith pollen- Variant V2 – drop of liquid was seeded withpollen together pistil, for a simulation of theconditions in vivo, relating to the stimulatingeffects that gynaeceum induces on startingof germination (on stigma) and then onpollinic tube development.- Variant V3 – liquid drop was seeded withpollen together remaining emptied anthersfor to have in view these possible negativeprocesses.Slides with media were kept at on averagetemperatures 17°C to 20°C in wet atmosphereso that the liquid medium doesn’t evaporate andthus it is maintained constant concentration inboric acid and sucrose.To reduce the risk of environmentalcontamination and to avoid the deterioration ofgermination medium, all the tools with whichthey were working, including filter paper andcultural medium were sterilized previously(Andrei and Paraschivoiu, 2003)..After sowing, the first laboratory tests weremade after a period of 5 hours of testing and 24hours.274