Scientific Papers Series B Horticulture

Figure 5. Macroscopic view of the colony ofMycosphaerella grossulariae (Auers.) Lind.CBS 235.37on PDA medium after 7 days (a), after 10 days (b) andafter 45 days (c ) from inoculation of the strain ofTrichothecium roseumAfter 7 days from inoculation of the antagonisticfungus Gliocladiumroseum, internalradius of its colony became in the neighborhoodsthe phytopathogenic fungus colony(Figure 6a). After 10 days the contact lineappeared as an obviously curve with theconcavity oriented to the pathogen; this ischaracteristic of the relationship between thetwo fungal colonies among which there aredifferences in growth rate (Figure 5b). Betweenthe two colonies being in the same Petri dishappeared a small area of sparse mycelium,which persisted throughout the 45 days ofexperimentation and it marked a inhibition ofthe pathogen exercises by saprophytic fungus(Figure 5b, Figure 5c). From this moment, theinternal radius of the pathogen was inhibited inits growth and remained at the value of 5.35mm, while the outer radius continued to growuntil the colony of Mycosphaerellagrossulariaewas completely surrounded by saprophyticfungus colony and the pathogen growth wascompletely inhibited (Figure 6c).pathogenic fungus was partially surrounded bythe colony of the saprophytic fungus andcontinued to grow only by external radius(Figure 7b.). At the end of the experiment,phytopathogenic colony was covered by that ofthe antagonistic (Figure 7c).Figure 7. Macroscopic view of the colony ofMycosphaerella grossulariae (Auers.) Lind.CBS 235.37on PDA medium after 7 days (a), after 10 days and after45 days (c ) from inoculation of the strain of EpicoccumnigrumTrichoderma viride fungus grew very quicklyover phytopathogenic fungus colony, so that at7 days after inoculation antagonistic fungusinvaded the colony, entirely covering the Petridish and determine to cease growth bothinternal and external radius (Figure 8a, Figure8b). Between the two colonies, pathogen’s andantagonist’s, the mycelium of fungus Trichodermaviride was sparse, sign that phytopathogenicfungus present in the vicinity mayhave an inhibitory effect on the fungus . At theend of the experiment, the Petri dish appearedtotally covered by mycelium of the fungus T.viride, except for those inhibition zones thatformed in the right of the two colonies thatinteracted (Figure 8c).Figure 6. Macroscopic view of the colony ofMycosphaerella grossulariae (Auers.) Lind.CBS 235.37on PDA medium after 7 days (a), after 10 days and after45 days (c ) from inoculation of the strain of GliocladiumroseumAfter 7 days the contact zone between colonyof the fungus Epicoccumnigrum and Mycosphaerellagrossulariaecolony became a curvewith the concavity oriented towards pathogenfungus which had a slower growth, and theinner radius was ceased growing (Figure 7a).After 10 days, the white mycelium of theFigure 8. Macroscopic view of the colony ofMycosphaerella grossulariae (Auers.) Lind.CBS 235.37on PDA medium after 7 days (a), after 10 days and after45 days (c ) from inoculation of the strain ofTrichoderma virideThe average values of the percent of growthinhibition calculated after 7 days allowed assessmentof inhibition as in the categories 4 forTrichoderma viride, 3 level for Trichotheciumroseum and Epicoccumnigrum and only 1 levelfor the strain of Gliocladiumroseum (Table 4,Table 9, Figure 10).433