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Scientific Papers Series B Horticulture

Scientific Papers Series B Horticulture

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Figure 6. Mature pollen during germination, in cherryBoambe de Cotnari variety , Ob.10x, Oc.10xFigure 7. Dynamic pollen tube length (PTL ) for 3varieties of cherry in variants (v1,v2.v3) with 20%sucroseCONCLUSIONSAs a result of these experiments conducted in2011, in which has been evaluated thebiological value of the pollen at 3 varieties ofRomanian cherry, we conclude the following:The pollen maturation went in normalphysiological conditions, undisturbed inaccordance with the evolution of relativelymild weather winter 2011.Tetrades and microspores had a normal aspectfor the development phasem.Mature pollen showed viability between 60%and 80% and germination 18% - 45%.The best germination has been on liquidmedium with sucrose 20% and (H 3 BO 3 ) 0,01%.Boambe de Cotnari cultivar is in the top, bothin viability (80%) and in germination (48%)271and variety Daria is in the last place with 60%viability and 18% maximum germination.The best germination of all varieties wasobtained in variant (v2/medium + pollen +pestle) with 20% sucrose. Thus confirming thestimulant role of pestle in triggering ofgermination and pollen tube growth.The poor germination was v 3 with 20%sucrose and v 3 with 15% sucrose in allvarieties. Version 3 (v 3 /medium + anthers)usually has a minimum value due to thenegative influence of environmental anthertissue on germination.For version (v 1 ) 20% sucrose, germination wasequivalent to (v 1 ) 15% sucrose and and hadmoderate values. Version 1 (v 1 /medium +pollen) can be considered indicative value forpotential germination of pollen specificgranules in the absence of pistle influence.PTL max. was recorded in variety Boambe deCotnari (400 ) also in the version (v2) on anaverage of 20% sucrose.The 3 Romanian varieties cherry have broughtforth the specific potential of each.It confirms good germination (18-45%) and thecorresponding binding undisturbed weatherconditions during flowering andmicrosporogenesis.REFERENCESAndrei M., Paraschivoiu R., 2003. MicrotehnicaBotanic. Editura Niculescu. Bucureti, p. 120-223.Andrei M., Rdulescu D., 1972. Caiet pentru tehnicapreparrii i conservrii materialului biologic–Tehnica obinerii preparatelor microscopice. (Fac.Biologie) Univ.Bucuresti. p. 1-83.Balan V., 2008. Caisul i caisele. Editura CeresBucuresti.Baciu E., 1971. Contributii la studiul microsporogenezeila unele specii din subfamilia prunoideae. Lucr.St.seria B XIV 1971. <strong>Horticulture</strong> IANB. Bucuresti, p.241-245.Blidariu A., Iordnescu O., Micu R., Drgunescu A.,2008. Researches concerning pollen’s germinationand viability of some sour cherries varieties in theplain area of Banat. Bulletin <strong>Horticulture</strong> UASVM.Bucuresti. vol. 65(1), p. 505-507.Bordeianu T., Tarnavschi I., Radu I. F., Bumbac E.,Botez M., Andrei M., 1961. Studiul privind repausulde iarna si pragul biologic la mugurii floriferi de cais.Revista Studii si cercetari. Seria biologie, 4.Butac M., Militaru M., 2006. Fertilitatea i germinareapolenului la genotipuri de mr, pr, prun, cire i

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