LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

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OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS) Test Substances: in simulated receiving water and soil environments at rates similar to that of sulfophenyl carboxylate (SPC) intermediates of a standard LAS. 14 C-benzene ring labeled C12 LAS (97.5% radiochemical purity); 14 C-benzene ring labeled iso-LAS of the following types (IA, 97.8% purity; IB, 77.6% purity; IIA, 94.7% purity; IIB, 97.5% purity); 14 C-benzene ring labeled DATS (97.3% purity), plus the non-labeled versions of the same. Reference: Nielsen, A.M., Britton, L.N., Beall, C.E., McCormick, T.P. and Russell, G.L. 1997. Biodegradation of coproducts of commercial linear alkylbenzene sulfonate. Environ. Sci. Technol. 31:3397-3404. Reliability: 2 Valid with restrictions (y) Type: aerobic [X]; anaerobic [ ] Method: OECD 301E. The study was designed to investigate the biodegradation of a relatively high iso-branched form of commercial LAS. The test was a prolonged batch-biodegradation experiment in which the material is kept “alive” for 80 days and in which the test compound present in a mineral salts medium is the sole carbon source. An enriched level of bacterial biomass, three times the amount recommended, was added at the test start using an inoculum obtained from the settled supernatant slurry solution of a fertile soil, without any previous exposure to the test compound. LAS was maintained by adding about 0 mg/L of fresh substance every four days for 80 days. After 80 days the test solution was sampled, centrifuged, sterilized with HgCl2 solution and analyzed with a chemical specific HPLC method with fluorescence detection. Results: Results indicate a residual LAS amount of 1.5 mg/L and SPC intermediate amount of 28.7 mg/L at the end of the 80 day study. Four distinct SPCs originating from the linear components of LAS were formed from the biodegradation experiment, and made up most of the organic residue. No evidence of structures related to the iso-branched material was found in the residue, therefore no accumulation of these materials is indicated. The isobranched component of LAS and the corresponding SPCs mineralized at rates as fast as the linear components. Test Substances: Commercial LAS (HF type) with a C10-C13 alkyl chain and a linearity of about 93%, with a low DATS content (
OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS) 28 day = 83% 29 day = 85% Method: OECD Test Guideline 301B and EC Directive 92/69/EEC C.4-C. Modified Sturm Test.. The test substance was added to a defined liquid mineral medium which was inoclulated with an activated-sludge inoculum and aerated at 19.7-21.9°C (mean 21.1°C). The inoculum used was activated non-adapted sludge from the Marl-Ost municipal sewage treatment plant. The inoculum had a bacterial count of 81 x 10 4 CFU/mL as determined by the Koch pour-plate method. The CO2 released was bound in the form of sodium carbonate in sodium hydroxide solution. Samples were collected and analyzed in duplicate for bound CO2 by TIC analysis after 0, 2, 5, 9, 12, 14, 21, 28 and 29 days. Sodium benzoate was used as a suitable control substance to monitor the activity of the inoculum. On the 29 th day, residual dissolved CO2 was expelled by acidification. GLP: Yes [X] No [ ] ? [ ] Test substance: Marlon A 365 WEL 6859 (CAS #68411-30-3) C10-13 LAS, average alkyl chain length = C11.6; Activity: 65% Remarks: LAS is readily biodegradable. The 10-day window criterion was fulfilled. The control substance (sodium benzoate) showed 89% degradation after 29 days. This is a key study for ready biodegradability (see SIAR Table 4). Reference: Enste-Diefenbach, R.. 2002. Marlon A 365 WEL 6859: Determination of biodegradability in the modified Sturm test.. Infracor GmbH Analytical Tehcnical Services, Report ST-204/02. Reliability: 1 Valid without restriction 3.6 BOD5, COD OR RATIO BOD5/COD BOD5 Method: DIN 38H03, Part 1. Bestimmung des biochemischen Sauerstoffbedarfs in 5 Tagen nach den Verduennungsprinzip. Value:
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LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

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