LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
Results: There was no significant difference in the incidence of chromosomal
aberrations between any of the groups given LAS and the negative control
group.
Control Group: Concurrent no treatment, positive and historical controls were used.
Method: Chromosomal aberrations were examined 6, 24, 48 hours after
administration. Mytomycin C was used as a positive control and
appropriately induced severe chromosomal aberrations.
GLP: Yes [ ] No [X] ? [ ]
Test substance: C10-14 LAS, sodium salt (CAS #69669-44-9); average alkyl chain length
(based on LAS SIDS Consortium Survey, 2000) = 11.7
Remarks: Besides the pure LAS, commercial preparation containing 19% LAS and
another containing 17.1% LAS were given to mice as single doses only by
gavage at 800, 1600 or 3200 mg/kg bw d and 1000, 2000 or 4000 mg/kg bw
d, respectively. The highest doses were 50% of the respective LD50 values.
No significant differences in the incidence of chromosomal aberrations were
observed in any LAS treatment relative to the controls. Information as cited
in the IPCS document.
Reference: 1) European Commission. 2000a. Benzenesulfonic acid, C10-13-alkyl derivs.,
sodium salts. Year 2000 CD-ROM edition.
2) Inoue, K., Shibata, T., Hamano, Y., Oda, Y., Kuwano, A., Yamamoto, H.,
Mitsuda, B. and Kunita, N. 1977. In vivo cytogenetic tests of some synthetic
detergents in mice. Ann Res. Osaka Prefect Inst. Public Health. 8:17-24 (in
Japanese); cited in: IPCS (1996); Environmental Health Criteria 169: Linear
Alkylbenzene Sulfonates (LAS) and Related Compounds. WHO, Geneva,
Switzerland.
Reliability: 4 This study is assigned a reliability score of 4 because the original report
was not available for review. However, the study was evaluated by IPCS
prior to inclusion in their criteria document
(b)
Type: Mammalian bone marrow cytogenetic assay
Species/strain: rat (Wistar, SD); mouse (ICR)
Sex: Female [ ]; Male [X]; Male/Female [ ]; No data [ ]
Administration: oral feed
Exposure period: 9 months
Doses: 0.9% (450 mg/kg bw d in rats; 1170 mg/kg bw d in mice)
Results: There were no significant differences in the incidences of chromosomal
aberrations between the experimental and control groups.
Method: Groups of 5 male Wistar rats, Sprague-Dawley rats, and ICR mice were
given a diet containing 0.9% LAS for 9 months after which the chromosomes
of the bone marrow cells were examined.
GLP: Yes [ ] No [X] ? [ ]
Test substance: LAS
Remarks: Information as cited in the IPCS document.
Reference: 1) European Commission. 2000a. Benzenesulfonic acid, C10-13-alkyl derivs.,
sodium salts. Year 2000 CD-ROM edition.
2) Masabuchi, M., Takahashi, A., Takahashi, O. and Hiraga, K. 1976.
Cytogenetic studies and dominant lethal tests with long term administration
of butylated hydroxytoluene (BHT) and linear alkylbenzene sulfonate (LAS)
in mice and rats. Ann. Rep. Tokyo Metrop. Res. Lab. Public Health. 27:100-
104 (in Japanese); cited in: IPCS (1996); Environmental Health Criteria 169:
Linear Alkylbenzene Sulfonates (LAS) and Related Compounds. WHO,
Geneva, Switzerland.
Reliability: 4 This study is assigned a reliability score of 4 because the original report
was not available for review. However, the study was evaluated by IPCS
prior to inclusion in their criteria document.
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