LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
Duration of the test: sacrifice at day 29 of pregnancy
Doses: 0.2, 2, 300, 600 mg/kg
Control Group: Yes; concurrent
NOAEL maternal
toxicity: 2 mg/kg bw
NOAEL teratogenicity: 2 mg/kg bw
GLP: Yes [ ] No [X] ? [ ]
Test substance: LAS
Results: Maternal toxicity:
At 300 and 600 mg/kg severe maternal toxicity was observed resulting in
body weight loss and associated with diarrhoea, anorexia, and cachexia prior
to death.
Teratogenicity:
At doses with no maternal toxicity, no differences were observed among the
dose groups and the control group with respect to number of litters, viable
young, litter weight, foetal weight, embryonic deaths, implantations and post
implantation embryonic loss. At these doses the incidences of major
malformations and minor abnormalities were not affected. Higher doses
resulted in total litter which was considered to be a secondary effect due to
the maternal toxicity. Since there were no survivors, malformations and
anomalies were not assessed at these doses.
Remarks: Information as cited in the IUCLID Data Sheet and the IPCS document.
Reference: Palmer, A.K., Readshaw, M.A. and Neuff, A.M. 1975a. Assessment of
the teratogenic potential of surfactants (Part I) – LAS, AS and CLD.
Toxicology 3:91-106.
Reliability: 2 Valid with restrictions
(g)
Species/strain: New Zealand White rabbit
Sex: Female [X]; Male [ ]; Male/Female [ ]; No data [ ]
Administration: intragastric intubation
Duration of the test: 29 days
Exposure period: day 6 to day 18 of pregnancy
Frequency of treatment:daily
Doses: 0.2, 2.0, 300, 600 mg/kg
Control group: Yes [X]; No [ ]; No data [ ];
Concurrent no treatment [X]; Concurrent vehicle [ ]; Historical [ ]
NOAEL Maternal
Toxicity: 2.0 mg/kg
NOAEL teratogenicity: 2.0 mg/kg
Results: Maternal toxicity: Daily assessment of bodyweight change and pregnancy
rate determined that at 0.2 and 2.0 mg/kg the treatment did not adversely
affect parent animals. At 300 and 600 mg/kg parent animals showed signs of
severe anorexia, diarrhoea, weight loss and death. Respective mortality rates
were 85 and 100% and autopsy consistently revealed changes in the
gastrointestinal tract.
Pregnancy/litter data: The influence of maternal toxicity restricted assessment
of effect on litter parameters to animals treated at 0.2 and 2.0 mg/kg. At
these two dosages there were no adverse effects on litter parameters, as
assessed by litter size and fetal loss, litter and mean pup weights.
Teratogenicity: Also at these two dosages there were no adverse effects on
embryonic and fetal development, as assessed by the incidence of major and
minor malformations, minor anomalies and skeletal variants.
Method: Thirteen rabbits were mated on a one-to-one basis with males of proven
fertility. The does were then injected intraveneously with 10 i.u. luteinizing
hormone to ensure that ovulation occurred. The rabbits were identified by an
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