LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

More documents

Recommendations

Info

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS) each animal was wrapped with plastic strips to avoid contamination and retard evaporation for the 24 hour exposure period. Observations were made over a period of seven days for irritation. The data was scored according to the method of Draize, Woodard and Calvery (Journal of Pharm. and Exp. Therapeutics, Volume 82, December 1944). GLP: Yes [ ] No [X] ? [ ] Test substance: Sodium sulfonate of linear alkylbenzene (Alkylate-215); C9 1%, C10 16%, C11 43%, C12 40%, C13 1%, C14
OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS) Method: OECD Guideline 405. Nine rabbits received a 0.1 mL aliquot of P-500 N-Na placed into the lower everted lid of one eye per animal. For three rabbits the eyelids were then gently held together for one second before releasing. For three other rabbits the eyes were irrigated with water for 5 minutes following a 4-second exposure. For the remaining three rabbits the eyes were irrigated for 5 minutes following a 30-second exposure. Eyes were examined after 1 hour and 1, 2, 3, 4, 7, 14 and 21 days after exposure. Grading was performed using the standard scoring system. GLP: Yes [X] No [ ] ? [ ] Test substance: Alkylbenzene sulfonate, sodium salt (designated as P-500 N-Na). Activity 47%. Average alkyl chain length = C11.2. Clear yellow liquid. Remarks: The following results were noted: 1) Three animals without any rinsing: averaged irritation scores (24, 48, 72 hours) for each animal: cornea 2.3, 1.7, 2; iris: 1.3, 0, 0; conjunctivae redness: 3, 1.7, 2; conjunctivae chemosis: 3, 2, 2. In the first animal the effects were persistent at day 14. 2) Three animals with rinsing for five minutes following a 30 second exposure: averaged scores: cornea 0.7, 1, 1.3; iris: 0, 0.7, 0.3; conjunctivae redness: 1.7, 2, 1.3; conjunctivae chemosis: 2, 1.3, 2. The eyes were normal 7 or 14 days after instillation. 3) Three animals with rinsing for five minutes following a 4 second exposure: averaged scores: cornea 0.7, 2.3, 0.7; iris: 0, 0, 0; conjunctivae redness: 1.7, 1.7, 1; conjunctivae chemosis: 1.3, 2, 1. The eyes were normal 7 days after instillation. Findings lead to a definition of irritating for LAS at 47% applied without rinsing, irritating (even if with lower effects, mainly as cornea opacity and conjunctivae redness) with rinsing after 30 second of exposure, and not irritating with rinsing after 4 second of exposure. Overall, instillation of P-500 N-Na into the eyes of rabbits elicited positive responses in all animals. Irrigation of the eyes only slightly reduced the irritation potential. Reference: Liggett, M.P. and Parcell, B.I. 1986b. Irritant effects on the rabbit eye of P- 500 N-Na. Huntingdon Research Ctr Report No. 86570D/PEQ 10/SE. Reliability: 1 Valid without restriction (b) Species/strain: New Zealand albino rabbits Results: Highly corrosive [ ]; Corrosive [ ]; Highly irritating [ ]; Irritating [X]; Moderate irritating [ ]; Slightly irritating [ ]; Not irritating [ ] Method: OECD Guideline 405. Six rabbits were exposed to 0.1 ml of 50% LAS, which was placed into the conjunctival sac of one eye per animal. The eyelids were gently held together for one second and then released. No irrigation step was performed The eyes were examined at 1, 24, 48 and 72 hours and at 7 days and scored for irritation using the standard system. GLP: Yes [ ] No [X] ? [ ] Test substance: LAS (made from Sirene LAB); activity 50%; average alkyl chain length = C11.6 Remarks: Average irritation scores were 1.3, 1.0, 2.6, and 2.7 for the cornea, iris, conjunctival redness, and conjunctival chemosis, respectively. Effects were persistent to Day 6. This classifies LAS at 50% as irritating. Reference: Biolab SGS. 1989d. Acute eye irritation. Report No. 00428/13. Reliability: 2 Valid with restrictions (c) Species/strain: rabbit UNEP PUBLICATIONS 265
Page 1 and 2:
OECD SIDS LINEAR ALKYLBENZENE SULFO
Page 3 and 4:
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214: OECD SIDS LINEAR ALKYLBENZENE SULFO
Page 263: OECD SIDS LINEAR ALKYLBENZENE SULFO
Page 315 and 316:
Page 317 and 318:
Page 319 and 320:
Page 321 and 322:
Page 323 and 324:
Page 325 and 326:
Page 327 and 328:
Page 329 and 330:
Page 331 and 332:
Page 333 and 334:
Page 335 and 336:
Page 337 and 338:
Page 339 and 340:
Page 341 and 342:
Page 343 and 344:
Page 345 and 346:
Page 347 and 348:
Page 349 and 350:
Page 351 and 352:
Page 353 and 354:
Page 355 and 356:
Page 357:
show all

LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

Create successful ePaper yourself

Delete template?

Save as template?