LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
cycle. The NOEC normalized by van de Plassche et al. (1999) to C11.6 LAS
was 2.3 mg/L.
GLP: Yes [ ]; No [X]; ? [ ]
Test Substance: Marlon A 350 LAS (CAS #68411-30-3; Benzenesulfonic acid, C10-13- alkyl
derives., sodium salts, 25.7% activity)
Reference: Henkel KGaA, Biological Research and Product Safety/Ecology:
unpublished results (Test substance number Fi 5959).
Reliability: 2 Valid with restrictions. Duration of test considered too short for a chronic
study, therefore not included in SIAR Table 12A.
(b)
Type of test: static [ ]; semi-static [ ]; flow-through [X]; other [ ]
open-system [X]; closed-system [ ]
Species: Pimephales promelas (Fish, fresh water)
Exposure period: 30 day
Effect criteria: Fry survival
Results: NOEC = 1 mg/L
LOEC = 2 mg/L
Analytical monitoring: Yes [X] No [ ] ? [ ] HPLC
Methods: Two replicates of 100 egg-fry stage fathead minnows were exposed for 30
days to LAS under the following conditions: Hardness 41 mg/L as CaCO3;
pH 7.2; temperature 24 o C. The exchange rate was 1 to 3 volume
changes/day. Test chambers were 3500 mL volume. The studies were
conducted at EG&G Bionomics (now Springborn Smithers Laboratory).
GLP: Yes [ ] No [ ] ? [X]
Test substance: Commercial C10-13 LAS, sodium salt (CAS #68411-30-3); C10 5%, C11 27%,
C12 53%, C13 13%; 2-phenyl 23%.
Remarks: Carboxylated intermediates formed in the biodegradation of LAS exhibit
toxicity several orders of magnitude less than LAS; LC50 values were >144
mg/L and >52 mg/L for sulfophenyl butarate and sulfophenyl undecanoate,
respectively. NOEC based on fry survival. Egg hatchability and fry growth
were less sensitive. This is a key study for chronic aquatic toxicity to fish
(see SIAR Table 12).
Reference: Swisher, R.D., Gledhill, W.E., Kimerle R.A. and Taulli, T.A. 1978.
Carboxylated intermediates in the biodegradation of linear alkylbenzene
sulfonates (LAS). VII International Congress on Surface Active Substance,
Proceedings, Moscow, 1976 4:218-230.
Reliability: 2 Valid with restrictions
(c)
Type of Test: Static [ ]; semi-static [X]; flow-through [ ]
Open-system [X]; closed-system [ ]; not stated [ ]
Species: Poecilia reticulata (Fish, Guppy, fresh water)
Exposure Period: 28 days
Effect Criteria: Mortality, behavior, and growth
Results: NOEC = 3.2 mg/L
LOEC = 10 mg/L
Analytic Monitoring: Yes [ ]; No [X]; ? [ ]
Method: Fish were 3-4 weeks old at test initiation. Fifty fish were used per group.
Temperature was maintained at 23 ± 2°C. The test volume (10-L per
chamber) was renewed three times per week. Circadian lighting (16:8
light:dark) was used. Fish were fed a Tetramin/Tetraphyll mixture.
Dissolved oxygen, water hardness and pH were measured during the study.
GLP: Yes [ ]; No [ ]; ? [X]
Test Substance: LAS, 42.4% activity (C8