LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
skeletal variants were not statistically significant with the exception of a
marginal retardation of sternebral ossification at 600 mg/kg.
Method: After overnight mating, the rats were randomly allocated to five groups
which included one control group and four different treatment groups. LAS
was prepared daily as a series of graded aqueous solutions. Animals in all
groups were dosed orally at the standard volume of 1.0 mL/100 g. Control
animals were dosed in a similar manner with distilled water used as the
vehicle. The dams were observed daily for signs of toxicity and weighed on
days 1, 3, 6, 10, 14, 17 and 20 of pregnancy. On day 20, the rats were killed
by CO2 euthanasia. Their ovaries and uterine contents were examined
immediately for number of copora lutea, number of viable young, number of
resorption sites, litter weight, and fetal abnormalities.
GLP: Yes [ ] No [X] ? [ ]
Test substance: LAS (Na salt) as a slurry containing 64.0% w/v of active ingredient (Lion Oil
and Fat Co., Ltd.); average alkyl chain length (based on LAS SIDS
Consortium Survey, 2002) = C11.7-12.3.
Reference: Palmer, A.K. and Lovell, M.R. 1971a. Effect of LAS detergent on
pregnancy of the rat. Report No. 4331/71/487.
Reliability: 2 Valid with restrictions
(d)
Species/strain: Charles River Specific Pathogen Free mice of the CD-1 strain
Sex: Female [X]; Male [ ]; Male/Female [ ]; No data [ ]
Administration: Oral in distilled water
Duration of the test: 17 days
Exposure period: day 6 to day 15 of gestation
Frequency of treatment: daily
Doses: 0.2, 2.0, 300, 600 mg/kg
Control group: Yes [ X ]; No [ ]; No data [ ];
Concurrent no treatment [X]; Concurrent vehicle [ ]; Historical [ ]
NOAEL Maternal: 2.0 mg/kg
NOAEL teratogenicity: 600 mg/kg
Results: Maternal toxicity: After examining parent animals, treatment at 300 and 600
mg/kg was linked with increased mortality (35% and 90% respectively) and
weight loss. At 600 mg/kg no dams bearing feasible young survived to
termination. Autopsy revealed the consistent occurrence of tympanites
sometimes associated with gastritis.
Pregnancy/litter data: The litter parameters were assessed by litter size, fetal
loss and litter weight, none of which were significantly affected by treatment
at any dosage. Mean pup weight was increased at 0.2 and 2.0 mg/kg.
Teratogenicity: Embryonic and fetal development was assessed by the
incidence of major malformations and minor visceral anomalies and the
distribution of skeletal variants. Any malformations and anomalities
observed were not dose related. Development was not significantly affected
at any dosage.
Method: After overnight mating, the mice were randomly allocated to five groups
which included one control group and four different treatment groups. LAS
was prepared daily as a series of graded aqueous solutions. Animals in all
groups were dosed orally at the standard volume of 0.06 mL/10 g. Control
animals were dosed in a similar manner with distilled water used as the
vehicle. The dams were observed daily for signs of toxicity and weighed on
days 1, 3, 6, 10, 14, and 17 of pregnancy. On day 17, the mice were killed by
cervical dislocation. Their uterine contents were examined immediately for
number of viable young, number of resorption sites, litter weight, and fetal
abnormalities.
GLP: Yes [ ] No [X] ? [ ]
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