LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
Test substance: LAS (Na salt), as a slurry containing 64.0% w/v of active ingredient (Lion
Oil and Fat Co., Ltd.); average alkyl chain length (based on LAS SIDS
Consortium Survey, 2002) = C11.7-12.3.
Reference: Palmer, A.K. and Lovell, M.R. 1971b. Effect of LAS detergent on
pregnancy of the mouse. Report No. 4330/71/486.
Reliability: 2 Valid with restrictions
(e)
Species/strain: Mouse/CD-1
Sex: Female [X]; Male [ ]; Male/Female [ ]; No data [ ]
Administration: gavage
Duration of the test: sacrifice at day 17 of pregnancy
Exposure period: days 6 - 15 of pregnancy
Frequency of treatment:daily
Doses: 0.2, 2, 300, 600 mg/kg bw d
Control group: Yes [X]; No [ ]; No data [ ];
Concurrent no treatment [X]; Concurrent vehicle [ ]; Historical [ ]
NOAEL Maternal
Toxicity: 2 mg/kg bw d
NOAEL teratogenicity: 300 mg/kg bw d
Results: Maternal toxicity:
Among parent animals treatment at 300 and 600 mg/kg bw d was associated
with increased mortality (35% and 90% respectively). At 300 mg/kg bw d
weight gain was retarded only during the first four days. No assessment
could be made at 600 mg/kg bw d, due to the high mortality rate. Necropsy
revealed a ubiquitous occurrence of tympanites, sometimes associated with
gastritis. Pregnancy rate was essentially comparable for all groups.
Teratogenicity:
At doses with no maternal toxicity, no differences were observed among the
dose group and the control group with respect to number of litters, viable
young, litter weight, foetal weight, embryonic deaths, implantations and post
implantation embryonic loss. At these doses the incidences of major
malformations and minor abnormalities were not affected. At doses with
maternal toxicity there was an increased foetal loss and reduced litter size
due almost entirely to total litter loss, which was considered to be a
secondary effect due to the maternal toxicity. The incidences of major
malformations was not affected; minor skeletal or visceral anomalies were
increased at 300 mg/kg.
Method: Twenty female mice were administered 0.2, 2.0, 300, or 600 mg/kg bw of
LAS by gavage at days 6-15 of gestation. All animals were sacrificed at day
17 of pregnancy.
GLP: Yes [ ] No [X] ? [ ]
Test substance: LAS
Remarks: The maternal NOAEL of 2 mg/kg bw d is considered very conservative
because the range (2-300 mg/kg bw d) was too wide.
Reference: Palmer, A.K., Readshaw, M.A. and Neuff, A.M. 1975a. Assessment of
the teratogenic potential of surfactants (Part I) – LAS, AS and CLD.
Toxicology 3:91-106.
Reliability: 2 Valid with restrictions
(f)
Species/strain: New Zealand white rabbit
Sex: Female [X]; Male [ ]; Male/Female [ ]; No data [ ]
Administration: Gavage
Exposure period: day 6 to 18 of pregnancy
Frequency of treatment: Daily
UNEP PUBLICATIONS 293