LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
LOAEL: 500 mg/kg bw d in diet
Results: In the mice given 0.6% in their diet, body weight gain was not suppressed,
but the weight of the liver increased in male and female mice. Enzymatic
examinations revealed significant decreases in LDH of the liver and in acid
phosphatase of the kidneys in the male mice. For mice given LAS in
drinking water, body weight was depressed at the highest dose for males and
females. This dose also elicited an increase in liver weight in females and
significant decreases in renal Na and K-ATPase.
Method: Groups of 8 or 9 mice were given diets containing LAS at concentrations of
0.6 and 1.8% or drinking water containing LAS at concentrations of 0.07,
0.2, and 0.6% for 9 months.
GLP: Yes [ ] No [X] ? [ ]
Test substance: LAS
Reference: 1) European Commission. 2000a. Benzenesulfonic acid, C10-13-alkyl derivs.,
sodium salts. Year 2000 CD-ROM edition.
2) Yoneyama, M., Mabuchi, Y., Ikawa, M., Kobayashi, H. and Ichikawa, H.
1976. Subacute toxicity of linear alkylbenzene sulfonate. Ann. Rep. Tokyo
Metr. Res. Lab. P.H. 27(2):105-112 (in Japanese); cited in: IPCS (1996);
Environmental Health Criteria 169: Linear Alkylbenzene Sulfonates (LAS)
and Related Compounds. WHO, Geneva, Switzerland.
3) HERA. 2002. HERA-LAS Human and Environmental Risk Assessment:
Linear Alkylbenzene Sulphonates, LAS. CAS No. 68411-30-3, Draft #6,
May 2002.
Reliability: 4 This study is assigned a reliability score of 4 because the original report
was not available for review. However, the study was evaluated by IPCS
prior to inclusion in their criteria document.
(k)
Species/strain: Rat: Charles River
Sex: Female [ ]; Male [ ]; Male/Female [X]; No data [ ]
Administration: oral feed
Exposure period: 2 years
Frequency of treatment:continuous in feed
Doses: 0.02, 0.1, 0.5% (10, 50, 250 mg/kg bw d)
Control group: Yes [X]; No [ ]; No data [ ];
Concurrent no treatment [ X]; Concurrent vehicle [ ]; Historical [X]
Results: Gross examination of all animals for pathology did not reveal any
abnormalities. No consistent dietary induced changes that could be
considered a toxic response were observed. Animals that showed significant
loss of weight, development of tumors, or other evidence of abnormalities
were sacrificed and tissues examined. The incidence of tumors and the
common incidental diseases were similar in all dieting groups. No treatmentrelated
adverse histological effects were observed in any of the tissue sections
examined.
Method: Four groups of Charles River weanling rats, divided by sex, were given 0.5,
0.1, and 0.02% LAS in their food for 2 years. Following completion of those
studies, five male and five female rats from each of the parental groups (F1b
and F2b) and all survivors were selected for necropsy. Livers and kidneys
were removed and weighed. Body weight and organ to body weight ratios
were recorded, and routine hematology and histology were performed.
Sections for histological examination were taken from the liver, kidney,
thyroid, trachea, esophagus, lung, heart, spleen, pancreas, adrenals, stomach,
small intestine, urinary bladder, gonads and mesenteric lymph nodes.
Weanling animals for the F3a generation were similarly treated.
GLP: Yes [ ] No [X] ? [ ]
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