LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
Results: Highly corrosive [ ]; Corrosive [ ]; Highly irritating [ ];
Irritating [X]; Moderate irritating [ ]; Slightly irritating [ ];
Not irritating [ ]
Classification: Irritating [X]; Not irritating [ ]; Risk of serious damage to eyes [ ]
Method: LAS solutions at 6 different concentrations ranging from 0.01% to 5.0% were
instilled in the eyes of rabbits (3 per group). The rabbits were observed for
168 hours after LAS application.
GLP: Yes [ ] No [X] ? [ ]
Test substance: LAS (chain length distribution C10-14; 80.9% of C11-13) (CAS #69669-44-9);
average alkyl chain length (based on LAS SIDS Consortium Survey, 2000) =
C11.7
Remarks: Information as cited in IPCS document. The 0.01% group showed no
reaction. Within 2 hours, the 0.05% group showed slight congestion and the
0.1% group showed considerable congestion or oedema, which disappeared
at 24 hours. In the group of 0.5% and higher, marked reactions such as
severe congestion an oedema, increased secretion, turbidity of the cornea,
and disappearance of the corneal reflex were observed for 24 hours. The
effects disappeared completely at 120 hours.
Reference: Iimori, M., Ogata, T. and Kudo, K. 1972. Eye irritation testing of surface
active agents in experimental animals. Jour. Jap. Oil Chem. Soc. 22:807-813
(in Japanese); cited in: IPCS (1996); Environmental Health Criteria 169:
Linear Alkylbenzene Sulfonates (LAS) and Related Compounds. WHO,
Geneva, Switzerland.
Reliability: 4 This study is assigned a reliability score of 4 because the original report
was not available for review. However, the study was evaluated by IPCS
prior to inclusion in their criteria document.
(g)
Species/strain: New Zealand albino rabbits
Results: Highly corrosive [ ]; Corrosive [ ]; Highly irritating [ ];
Irritating [ ]; Moderate irritating [ ]; Slightly irritating [ ];
Not irritating [X]
Method: OECD Guideline 405. Six rabbits were exposed to 0.1 ml of 1% LAS, which
was placed into the conjunctival sac of one eye per animal. The eyelids were
gently held together for one second and then released. No irrigation step was
performed The eyes were examined at 1, 24, 48 and 72 hours and at 7 days
and scored for irritation using the standard system.
GLP: Yes [ ] No [X] ? [ ]
Test substance: LAS (made from Sirene LAB); activity 1%; average alkyl chain length =
C11.6
Remarks: Average irritation scores were 0, 0, 0.1, and 0.1 for the cornea, iris,
conjunctival redness, and conjunctival chemosis, respectively. This
classifies LAS at 1% as not irritating.
Reference: Biolab SGS. 1984. Acute eye irritation. Report No. T3R/27.
Reliability: 2 Valid with restrictions
(h)
Species/strain: New Zealand albino rabbits
Results: Highly corrosive [ ]; Corrosive [ ]; Highly irritating [ ];
Irritating [ ]; Moderate irritating [X]; Slightly irritating [ ];
Not irritating [ ]
Methods: OECD Guideline 405. Six rabbits were exposed to 0.1 ml of 5% LAS, which
was placed into the conjunctival sac of one eye per animal. The eyelids were
gently held together for one second and then released. No irrigation step was
performed The eyes were examined at 1, 24, 48 and 72 hours and at 7 days
and scored for irritation using the standard system.
UNEP PUBLICATIONS 267