LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
Species/strain: mouse (DDY)
Sex: Female [ ]; Male [ ]; Male/Female [ ]; No data [X]
Administration: drinking water
Exposure period: 6 months
Frequency of treatment: Daily
Observation period: 2 months post exposure
Dose: 100 ppm in the drinking water (20 mg/kg bw d)
Control group: Yes [X]; No [ ]; No data [ ];
Concurrent no treatment [X]; Concurrent vehicle [ ]; Historical [ ]
Results: Atrophy of the golgi apparatus, degeneration of the mitochondria, and
increased appearance of lysosomes were observed. The severity of these
adverse effects were dependent on the length of the administration. After six
months, some cells showed degenerative cytoplasm and indications of cell
necrosis. Effects on the rough endoplasmatic reticulum were observed.
Some animals still showed cellular effects after the two months post
administration period while other animals showed full recovery. Given the
unknown significance of the effects for the health of the animals and the
reversibility of the effects, as well as the consistent lack of adverse effects in
other studies at similar or higher doses, the dose tested in this study is
considered a LOEL rather than a LOAEL.
Method: LAS was administered up to 6 months. The animals were sacrificed at 1, 2,
3, and 6 months. Some animals were observed an additional 2 months
without test substance administration. Liver slices were investigated using
electron microscopy.
GLP: Yes [ ] No [X] ? [ ]
Remarks: The reliability and usefulness of this study for risk assessment purposes is
limited. The study employed only a single dose (i.e., no dose response
information). In addition, there is the likelihood of dehydration of the
animals. Because of these study deficiencies, it was determined that it is
inappropriate to derive a LOAEL from this study.
Test substance: LAS (unspecified)
Reference: 1) European Commission. 2000a. Benzenesulfonic acid, C10-13-alkyl derivs.,
sodium salts. Year 2000 CD-ROM edition.
2) Watari, N., Torizawa, K., Kanai, M. and Suzuki, Y. 1977. Ultrastructural
observations of the protective effect of glycyrrhizin for mouse liver injury
caused by oral administration of detergent ingredient (LAS) J. Clin. Electron.
Microscopy 10:121-139 (in Japanese) cited in IPCS. 1996. Environmental
Health Criteria 169: Linear Alkylbenzene Sulfonates and Related
Compounds. World Health Organization, Geneva, Switzerland.
Reliability: 4 This study is assigned a reliability score of 4 because the original report
was not available for review. While the study was evaluated by IPCS prior
to inclusion in their criteria document, the deficiencies noted above make
its usefulness in risk assessment questionable.
(j)
Species/strain: mouse (ICR)
Sex: Female [ ]; Male [ ]; Male/Female [X]; No data [ ]
Administration: Oral feed or water
Exposure period: 9 months
Frequency of treatment: Daily
Dose: Diet: 0.6 and 1.8% (corresponding to 500 and 1000 mg/kg bw d).
Diet: Drinking water: 0.07, 0.2, and 0.6% (100, 250, and 600 mg/kg bw d for
males and 100, 250, and 900 mg/kg bw d for females)
Control group: Yes [X]; No [ ]; No data [ ];
Concurrent no treatment [X]; Concurrent vehicle [ ]; Historical [ ]
NOAEL: 250 mg/kg bw d in drinking water
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