LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
Concurrent no treatment [X]; Concurrent vehicle [ ]; Historical [X]
NOAEL Parental: = 0.5%. (350 mg/kg bw d)
NOAEL F1 Offspring: = 0.5% (350 mg/kg bw d)
NOAEL F2 Offspring: = 0.5% (350 mg/kg bw d)
Results: General reproduction including fertility gestation, parturition, neonatal
viability, lactation, and post-weaning growth was normal for all test groups
and did not deviate from the controls in each generation. No gross
abnormalities were noted. No definitive adverse effects due to the test
material were noted in the haematology and pathology.
Method: Na-LAS (chain length distribution C10-14) was fed for 84 days to 4 groups of
weanling rats (3 dose levels, plus control), each dose consisting of 50 animals
each of both sexes (PO-generation). When the P0 generation was 107-112
days old, 20 females from each dose group were mated with 20 males from
the same group and maintained together for 17 days. The first litters of each
generation (Fla- and F2a-generation) were sacrificed at 21 days of age. Ten
days after the final litter was sacrificed, all females were remated with
different males from the same group to obtain the F1b generation. From the
Flb-generation, 20 males and females of each group were selected at weaning
to continue their respective diets and to be used for further reproduction
studies. Reproduction studies on the F1b and F2b generations were started
when the rats were 80 to 85 days old, and were continued until the F3b
generation was weaned.
GLP: Yes [ ] No [X] ? [ ]
Test substance: Sodium salt LAS (C10-14), activity: 98.1% on an anhydrous basis (41.9%
active)
Reference: Buehler, E.V., Newmann, E.A., and King, W.R. 1971. Two year feeding
and reproduction study in rats with linear alkylbenzene sulfonate (LAS).
Toxicol. Appl. Pharmacol. 18:83-91.
Reliability: 2 Valid with restrictions
(b)
Type: Fertility [ ]; One-generation study [ ]; Two-generation study [ ];
Other [X] Three generation study
Species/strain: Charles River CD strain rats
Sex: Female [ ]; Male [ ]; Male/Female [X]; No data [ ]
Administration: diet
Exposure period: up to > 1 year
Frequency of treatment:continuous in diet
Premating exposure
period: male: 60 days, female: 60 days
Duration of the test: 3 generations
Doses: 0.08, 0.4, and 2.0% continuously administered throughout the three
generations (40, 200 and 1000 mg/kg bw d CLD [6.8, 3.4 and 170 mg/kg bw
d LAS]
Control group: Yes [X]; No [ ]; No data [ ];
Concurrent no treatment [X]; Concurrent vehicle [ ]; Historical [ ]
NOAEL Parental: 170 mg/kg bw d LAS (1000 mg/kg bw d CLD)
NOAEL F1 Offspring: 170 mg/kg bw d LAS (1000 mg/kg bw d CLD)
NOAEL F2 Offspring: 170 mg/kg bw d LAS (1000 mg/kg bw d CLD)
Results: General parental toxicity: There were no signs of malreaction to treatment
among parents and the incidence of sporadic deaths and total litter losses
were unrelated to dosage. Pregnancy rate and the duration of gestation were
unaffected. Food consumption and bodyweight changes showed no
consistent relationship to dosage over the three generations.
Toxicity to offspring: Examining litter parameters, statistically significant
differences were occasionally observed but they showed no consistent dosage
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