LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
protection criteria developed for these species should be adequate to protect
the aquatic macrophyte community without the need for additional testing.
Reference: Bishop, W.E. and Perry, R.L. 1981. Development and Evaluation of a Flow-
Through Growth Inhibition Test with Duckweed (Lemna minor). Aquatic
....... Toxicology and Hazard Assessment. ASTM STP 737. Branson, D.R.
and Dickson, K.L. (ed.).
Reliability: 2 Valid with restrictions
(q)
Type of Test: Static [X]; semi-static [ ]; flow-through [ ]
Open-system [ ]; closed-system [ ]; not stated [X]
Species: Chlamydomonas reinhardi (algae)
Exposure Period: not stated
Effect Criteria: Growth inhibition
Results: NOEC = 15 mg/L
LOEC = 20 mg/L
Analytic Monitoring: Yes [ ]; No [ ]; ? [X]
Method: LAS concentrations of 1, 5, 10, 15, 20 and 30 mg/L were prepared in
Deionized, sterilized double-distilled glass water. Algae were grown
in Bold’s medium. Cell counts were made using a spectrophotometer and
dry weight observations.
GLP: Yes [ ]; No [X]; ? [ ]
Test Substance: C11.2 LAS
Remarks: No morphological changes were observed. The growth rate was reduced at
LAS concentrations of 20 mg/L. Protein analysis indicated that higher
concentrations did affect protein synthesis. The NOEC normalized by van de
Plassche et al. (1999) to C11.6 LAS was 12 mg/L.
Reference: Dhaliwal, A.A., Campione, A., and Smaga, S. 1977. Effect of linear
alkylbenzene sulfonate (C11.2 LAS) on the morphology and physiology of
Plectonema boryanum and Chlamydomonas reinhardi. J. Phycol. 13:18.
Reliability: 4 Not assignable. This study was given a reliability score of 4 because the
document reviewed was an abstract.
(r)
Type of Test: Static [X]; semi-static [ ]; flow-through [ ]
Open-system [ ]; closed-system [ ]; not stated [X]
Species: Chlorella kessleri (algae)
Exposure Period: 15 days
Effect Criteria: Growth rate
Results: NOEC = 3.1 mg/L
LOEC = 10 mg/L
Analytic Monitoring: Yes [ ]; No [X]; ? [ ]
Method: EPA-600/9-78-018. Algal Assay Bottle Test. Determination of the
Inhibitory effect of water constituents on green algae, by William E.
Joseph C. Greene, and Tamotsu Shiroyama, Cornwallis Environmental
Research Laboratory, Corvallis, Oregon.
GLP: Yes [ ]; No [X]; ? [ ]
Test Substance: Marlon A 350, Benzenesulfonic acid, C10-13-alkyl derives., sodium salts
(CAS #68411-30-3); 25.7% activity.
Remarks: No morphological changes were observed. The growth rate was reduced at
LAS concentrations of 20 mg/L. Protein analysis indicated that higher
concentrations did affect protein synthesis. The NOEC normalized by van de
Plassche et al. (1999) to C11.6 LAS was 3.5 mg/L.
Reference: Henkel KGaA, Biological Research and Product Safety/Ecology,
unpublished results of study conducted in 1984; test substance Fi 5829.
UNEP PUBLICATIONS 196