LINEAR ALKYLBENZENE SULFONATE (LAS) - UNEP Chemicals

OECD SIDS LINEAR ALKYLBENZENE SULFONATE (LAS)
related trends over the three generations and were considered to be unrelated
to treatment. The incidence of malformations was unaffected by treatment.
Additional organ weight analysis, histopathology and skeletal staining of
representative young from the F3b generation revealed no changes that could
be conclusively related to treatment.
Method: CLD was administered in the diet of the rats and new batches of diet were
prepared each week. Males and females of each generation (Fo, F1b, and F2b)
were kept on their respective diets for 60 days. The mating period for the
first litter lasted 19 days. After the weaning of the first litters, approximately
10 days, the animals were re-mated and a second litter was produced. From
the second litters of the initial (Fo) and second (F1b) generations, 10 males and
20 females were selected from each group at weaning in order to form the
second and third (F2b) generations, respectively.
In the parent animals, observations of signs of reaction, mortalities, food
consumption, bodyweight change, pregnancy rate, mating performance, and
gestation period were made throughout the study. As soon as possible (< 12
hours) after birth, all young were counted, identified by toe amputation and
examined for external abnormalities. Up to day 21 post partum, animals
were examined daily for dead and abnormal young. Young of the first litters
and surplus young of the second litters were sacrificed and examined for
abnormalities internally and externally. Rats of the F3b generation were killed
at 3 weeks old and were also examined internally and externally for
abnormalities. For the F3b generation, tissue from the brain, liver, heart,
pituitary, spleen, thyroid, kidneys, thymus, adrenals, lungs, gonads, pancreas,
bladder, bone, bone marrow, sections of the stomach, and sections of the
small and large intestines were removed and examined.
GLP: Yes [ ] No [X] ? [ ]
Test substance: Commercial Light Duty liquid detergent (CLD) containing 17% LAS and 7%
alkyl ether sulfate (Lion Oil and Fat Co., Ltd.)
Reference: Palmer, A.K., Cozens, D.D., Batham, P., and Cherry, C.P. 1974. Effect of
CLD on reproductive function of multiple generations in the rat. Final
Report. Report No. LF010/731029.
Reliability: 2 Valid with restrictions
(c)
Type: Fertility [ ]; One-generation study [ ]; Two-generation study [ ];
Other [X] : 4-generation reproduction study
Species/strain: Rat/Wistar
Sex: Female [ ]; Male [ ]; Male/Female [X]; No data [ ]
Administration: drinking water
Frequency of treatment: Daily
Duration of the test: 4 generations
Doses: 0.1% (70 mg/kg bw d)
Control group: Yes [X]; No [ ]; No data [ ];
Concurrent no treatment [X]; Concurrent vehicle [ ]; Historical [ ]
NOAEL Parental: 70 mg/kg bw d
NOAEL F1 Offspring: 70 mg/kg bw d
NOAEL F2 Offspring: 70 mg/kg bw d
Results: The administration of LAS had no adverse effects on fertility, parturition,
gestation period, or lactation in any of the generations.
Method: Two groups of 20 rats of both sexes were given water containing LAS and
the reproductive performance was investigated for 4 generations. Five to 10
rats of both the control and the experimental group were sacrificed at 12
weeks for pathological examinations. For successive reproduction, 15 males
and 15 females produced by the first mating of rats were used.
GLP: Yes [ ] No [X] ? [ ]
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