The exposure and toxicity of pesticides to amphibians

Amphibians and pesticides
Relyea, R.A. (2005b) Some data for LC50 values that include the effect of
predatory stress are included in the ECOTOX data
but it was felt better to omit these.
Zaffaroni et al. (1978) Data for Maneb (Maneb 80) and Triturus cristatus
described as LC50 is in fact the concentrations at
which LT50 was measured (not LC50s).
After removing this data some 2124 LC50 values remained. Due to these issues, some of which were
found early on in the collation of the data, it was decided as far as possible to check all of the data
against the original source where this could be obtained. All values were assessed to determine if any
further data should be omitted. Reasons for omission included:
• test material not defined (e.g. formulation or technical material),
• formulation data with no % a.i. information,
• lack of replication (animals per dose),
• no information on number of dose levels used.
In some cases where no detailed information on number of dose levels was found, but a standard
method was used or other information indicated that sufficient doses were likely to have been used,
the studies were accepted. In some cases the source language allowed the values to be checked but
other details of the study could not be extracted. These values were also excluded. In total 737 values
were omitted leaving 1387 LC50 values. These were divided into tables depending on test period
duration (24h, 48h, 72h, 96h and ‘all other durations’).
The selected data for 96h studies is shown in Table 11. Data for other standard test durations (24, 48
and 72h) are shown in Appendices I to L. The data that was omitted is shown in Appendix M along
with the reasons they were not included.
Supporting publications 2012:EN-343 40
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