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The exposure and toxicity of pesticides to amphibians

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Pesticide %<br />

a.i.<br />

Test<br />

material<br />

Ekatin NR NR Rana<br />

temporaria<br />

Ekatin NR NR Rana<br />

temporaria<br />

Fenitrothion NR Form. Rana<br />

temporaria<br />

Fenitrothion NR Form. Rana<br />

temporaria<br />

Fenpropathrin<br />

(S)<br />

NR Tech. Rana pipiens<br />

pipiens<br />

Species Stage Temp.<br />

°C<br />

Exposure<br />

type<br />

Amphibians <strong>and</strong> <strong>pesticides</strong><br />

Supporting publications 2012:EN-343 89<br />

<strong>The</strong> present document has been produced <strong>and</strong> adopted by the bodies identified above as author(s). This task has been carried out exclusively by the author(s) in the context <strong>of</strong> a contract between the European Food<br />

Safety Authority <strong>and</strong> the author(s), awarded following a tender procedure. <strong>The</strong> present document is published complying with the transparency principle <strong>to</strong> which the Authority is subject. It may not be considered as an<br />

output adopted by the Authority. <strong>The</strong> European food Safety Authority reserves its rights, view <strong>and</strong> position as regards the issues addressed <strong>and</strong> the conclusions reached in the present document, without prejudice <strong>to</strong> the<br />

rights <strong>of</strong> the authors.<br />

Dur.<br />

(h)<br />

Dose<br />

levels<br />

Anim.<br />

/dose<br />

LD50<br />

(mg/kg)<br />

(95%<br />

CL)<br />

Comments Reference<br />

NR - - - - - 2480 Data is for females as<br />

reported in Gromysz-<br />

Kalkowska <strong>and</strong><br />

Szubar<strong>to</strong>wska (1993). No<br />

further details listed.<br />

NR - - - - - 2600 Data is for males as<br />

reported in Gromysz-<br />

Kalkowska <strong>and</strong><br />

Szubar<strong>to</strong>wska (1993). No<br />

Adult<br />

male<br />

(21-<br />

56g)<br />

Adult<br />

female<br />

(21-<br />

56g)<br />

Adult<br />

(20-<br />

30g)<br />

further details listed.<br />

NR SC 3 5 11 2400 Not clear whether data is<br />

for formulation or active<br />

ingredient, comparisons<br />

with other studies/species<br />

suggests active ingredient.<br />

Value based on Trevan's<br />

method. Value was<br />

2342mg/kg using the<br />

method <strong>of</strong> Reed <strong>and</strong><br />

Muench.<br />

NR SC 3 5 11 2220 Not clear whether data is<br />

for formulation or active<br />

ingredient, comparisons<br />

with other studies/species<br />

suggests active ingredient.<br />

Value based on Trevan's<br />

method. Value was<br />

2182mg/kg using the<br />

method <strong>of</strong> Reed <strong>and</strong><br />

20 SC 24 NR NR 0.27<br />

(0.12-<br />

0.63)<br />

Muench.<br />

Assumed data based on<br />

active ingredient. Animals<br />

per dose level not defined<br />

but 28-34 <strong>to</strong>tal used.<br />

Szubar<strong>to</strong>wska<br />

(1990)<br />

Szubar<strong>to</strong>wska<br />

(1990)<br />

Gromysz-<br />

Kalkowska<br />

<strong>and</strong><br />

Szubar<strong>to</strong>wska<br />

(1993b)<br />

Gromysz-<br />

Kalkowska<br />

<strong>and</strong><br />

Szubar<strong>to</strong>wska<br />

(1993b)<br />

Cole <strong>and</strong><br />

Casida (1983)

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