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History of Natto and Its Relatives (1405-2012 - SoyInfo Center

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yield tests, replicated tests, resource allocation for yield<br />

evaluation, techniques for plot management (planting, endtrimming,<br />

harvest). 5. Breeder seed production: Methods<br />

<strong>of</strong> purifi cation, timing <strong>of</strong> breeder seed production. 6.<br />

Commercial use <strong>of</strong> seed mixtures: Marketing <strong>of</strong> seed, seed<br />

yield, overcoming defi ciencies <strong>of</strong> high-yielding cultivars,<br />

stability <strong>of</strong> performance, other considerations.<br />

Concerning seed size: The seed size <strong>of</strong> widely grown<br />

soybean varieties ranges from about 12-18 gm per 100<br />

seeds. Soybeans weighing less than 10 gm per 100 seeds<br />

are preferred for natto, whereas soybeans weighing more<br />

than 22 gm per 100 seeds are preferred for various food<br />

uses. Verde, a variety with green seeds that weigh 32 gm<br />

per 100 seeds was developed for processing as a canned or<br />

frozen vegetable. Prize, a yellow-seeded variety weighing<br />

27 gm per 100 seeds, has been used for home gardens, <strong>and</strong><br />

for making roasted soy fl our <strong>and</strong> miso. Address: Iowa State<br />

Univ., Ames, IA.<br />

1210. Kanno, Akishige; Takamatsu, Haruki. 1987. [Studies<br />

on natto. IV. Changes in the volatile components <strong>of</strong> natto<br />

during manufacture <strong>and</strong> storage]. Nippon Shokuhin Kogyo<br />

Gakkaishi (J. <strong>of</strong> the Japanese Society <strong>of</strong> Food Science <strong>and</strong><br />

Technology) 34(5):330-35. May. (Chem. Abst. 107:114495).<br />

[17 ref. Eng; jap]<br />

• Summary: Acetoin <strong>and</strong> 2,3-butanediol were found to<br />

accumulate <strong>and</strong> reach a maximum in natto after 12 hours <strong>of</strong><br />

fermentation, after which time these amounts decreased as a<br />

result <strong>of</strong> the action <strong>of</strong> microorganisms. Prior to this period,<br />

the level <strong>of</strong> citric acid, the major organic acid <strong>of</strong> soybeans,<br />

<strong>and</strong> glucose released from sucrose, decreased noticeably.<br />

Acetic acid increased gradually during the latter half <strong>of</strong><br />

fermentation <strong>and</strong> showed irregular variations during storage.<br />

Propionic acid was detected from the middle <strong>of</strong> fermentation<br />

<strong>and</strong> its increase was affected by storage temperature. 3<br />

branched-chain fatty acids (iso-butyric, 2 methylbutyric,<br />

<strong>and</strong> 3-methylbutyric acids) were detected after 12 hours<br />

<strong>of</strong> fermentation <strong>and</strong> increased thereafter. During storage at<br />

25 degree C, contents <strong>of</strong> these acids increased markedly.<br />

However no such change was observed during storage at 5 or<br />

15 degree C. Address: Asahi Shokuhin Co. Ltd., 180-2 Wado,<br />

Ushibori-machi, Namekata-gun, Ibaraki, 311-24, Japan.<br />

1211. Katagiri, Mitsuaki; Shimizu, S.; Kaihara, H.; Katagiri,<br />

C. 1987. [Determination <strong>of</strong> esterifi ed <strong>and</strong> free carboxylic<br />

acids in the various kinds <strong>of</strong> natto by gas chromatography].<br />

Nippon Shokuhin Kogyo Gakkaishi (J. <strong>of</strong> the Japanese<br />

Society <strong>of</strong> Food Science <strong>and</strong> Technology) 34(5):305-08. May.<br />

(Chem. Abst. 107:174685). [9 ref. Jap; eng]<br />

• Summary: Free <strong>and</strong> esterifi ed fatty <strong>and</strong> organic acids in<br />

various samples <strong>of</strong> natto (9 types <strong>of</strong> itohiki-natto <strong>and</strong> 6 types<br />

<strong>of</strong> tera-natto [fermented black soybeans]) were determined<br />

by a modifi ed st<strong>and</strong>ard method <strong>and</strong> compared with those <strong>of</strong><br />

common miso. Ethyl esters <strong>of</strong> fatty acids present in large<br />

© Copyright Soyinfo <strong>Center</strong> <strong>2012</strong><br />

HISTORY OF NATTO AND ITS RELATIVES 380<br />

quantities in miso were not detected in itohiki-natto, but<br />

found in small quantities in tera-natto. Lactic acid was the<br />

dominant organic acid in hama-natto, (470.1 mg/100 gm)<br />

<strong>and</strong> in tera-natto (26.2 mg/100 g) <strong>and</strong> was not detected in<br />

either itohiki- or hoshi-natto. Average contents <strong>of</strong> the major<br />

volatile organic acids in itohiki-natto were (mg/100 gm):<br />

acetic acid, 124.7; propionic acid, 28.4; iso-butyric acid,<br />

44.1; <strong>and</strong> iso-valeric acid, 46.7. The last 2 acids, which gave<br />

unfavorable odor at higher concentrations, were found in<br />

small amounts in hama-natto <strong>and</strong> tera-natto. Address: Dep.<br />

<strong>of</strong> Domestic Science, Iida Women’s Junior College, Matsuo<br />

610, Iida-shi, Nagano 395, Japan.<br />

1212. Kim, Bok-Ran; Han, Y.B.; Park, C.H. 1987.<br />

[Changes <strong>of</strong> free sugar <strong>and</strong> free amino acid during the<br />

natto fermentation used by Bacillus subtilis S.N.U. 816].<br />

Hanguk Nonghwa Hakhoe Chi (J. <strong>of</strong> the Korean Agricultural<br />

Chemical Society) 30(2):192-97. June. (Chem. Abst.<br />

107:19743). [39 ref. Kor; eng]<br />

• Summary: The free sugars examined were fructose,<br />

glucose, sucrose, <strong>and</strong> maltose. Remarkable increases in the<br />

levels <strong>of</strong> fructose <strong>and</strong> glucose were observed after 4 hours<br />

<strong>of</strong> fermentation. After that, however, their contents were<br />

reduced to a very low level as fermentation continued. The<br />

sucrose content dropped dramatically to about the 10% level<br />

<strong>and</strong> stayed low thereafter.<br />

The free amino acid content <strong>of</strong> natto during 20 hours<br />

<strong>of</strong> fermentation was twice as high as that <strong>of</strong> unfermented<br />

soybeans.<br />

Sensory evaluation showed that 20 hours <strong>of</strong><br />

fermentation at 43ºC produced the best quality natto based<br />

on taste, odor <strong>and</strong> color.<br />

Contains 5 tables <strong>and</strong> 1 graph. Address: 1-2. Dep. <strong>of</strong><br />

Home Economics Education, Korea Univ.; 3. Sampyo Foods<br />

Co., Ltd. Lab. All: Seoul, Korea.<br />

1213. Torii, Yasuko. 1987. New developments with tempeh<br />

in Japan (Interview). Conducted by William Shurtleff <strong>of</strong><br />

Soyfoods <strong>Center</strong>, July 1. 2 p. transcript.<br />

• Summary: Torigoe-Seifun stopped making tempeh in<br />

mid-1987. They had a hard time popularizing it. Marusan’s<br />

tempeh is not selling well. They, too, are thinking <strong>of</strong><br />

stopping. Mr. Kanasugi <strong>and</strong> the <strong>Natto</strong> Assoc. are trying<br />

to cooperate with Marusan. They suggest selling “tempeh<br />

miso,” made as follows: Mix 10 kg tempeh, 10 kg barley<br />

koji or rice koji, <strong>and</strong> 1 kg salt. Grind in a meat grinder <strong>and</strong><br />

let st<strong>and</strong> at room temperature in the summer for 1 month.<br />

The result is a sort <strong>of</strong> Finger Lickin’ Miso. Barley koji works<br />

best, <strong>and</strong> the resulting tamari is delicious. Mr. Kanasugu<br />

hopes some miso maker will make this. Only Mr. Kanasugi<br />

is doing tempeh research. He makes this miso in his<br />

restaurant daily. He also makes a spread like peanut butter.<br />

Mr. Ose is still making tempeh but his business, Udai, is not<br />

doing well. No one is left at the Ministry <strong>of</strong> Agriculture with

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