Untitled - Stiftung Tierärztliche Hochschule Hannover

7 Summary
7 Summary
Flavia Colombrino:
Investigations for evaluation of the hygiene conditions at aquaculture establishments
in Lower Saxony with respect to hygiene regulations of the European Union
The present study aimed in evaluation of the hygiene conditions in fishprocessing
aquaculture establishments in Lower Saxony, the detection of hygiene-relevant and
pathogenic microbes on furnishings as well as on working devices and their
surroundings. In addition, a preliminary used sampling protocol should be tested to
be practically and suiteable for the control of hygienic conditions after succeeded
cleaning and disinfection programmes.
Furthermore, pre-, intermediate- and endproducts of aquacultured fish were
examined microbiological, which gave additional informations to the process hygiene.
In the course of this research 15 Lower Saxony fishprocessing enterprises were
sampled between May 2007 and September 2008 for three times.
In total 453 tests, of it 332 swab samples (wet-dry-swab technique) as well as 121
samples of fish (rainbow trout-, eel- and catfish products) were taken and examined
regarding 9 different microbiological parameters referred to the official collection of
analysis methods according to § 64 of the German Food and Feed Code (LFGB).
The examination spectrum included the determination of the total aerobic plate count
at 25°C and 30°C, the quantitative detection of Enterobacteriaceae (aerobic and
anaerobic), Listeria spp., Pseudomonas spp., coagulase positive staphylococci and
qualitative detection of Aeromonas hydrophila and Listeria monocytogenes.
All sampling points of the sampling protocol showed high numbers of bacteria counts
in reference to the microbiological parameters total aerobic plate count at 25°C and
30°C and Pseudomonas.
The highest bacterial counts were determined in the group samples sluice, in
particular slaughter room – sluice regarding the parameter total aerobic plate count at
30°C (6.32 ± 1.27 CfU/sampling point) as well as in the category slaughter-brush,
especially hand-slaughtering brush for the microbiological parameter total aerobic
bacterial count at 25°C (4.92 ± 1.77 CfU/sampling point).
162