ICRISAT Archival Report 2006 - The seedlings of success in the ...

Characterization of variability in Fusarium udum : We also initiated work on characterization of variability in F.
udum. Eleven isolates of F. udum from nine locations in six states [Akola, Badnapur (Maharashtra); Bangalore,
Gulbarga (Karnataka); Khargone (Madhya Pardesh), Muradnagar (Meerut), Varanasi (UP), Warangal and
ICRISAT-Patancheru (Andhra Pradesh) all in India] were collected and single spore cultures were obtained using
standard mycological techniques. All the single spore isolates once again were passed through common wilt
susceptible cultivar ICP 2376 by following root dip inoculation technique, and aggressive isolates were stored at 4 0
C in the laboratory. Since the collection of the isolates did not represent the entire country, we continued to collect
the isolates from pigeonpea growing areas. During the current crop season, wilt infected samples were collected
from 9 locations (Yenagandla, Kulcharam, Jogipet, Andole, and Shivampet in Andhra Pradesh; Aurad in Karnataka;
and Dhuki, Osmanabad, and Parbhani in Maharashtra, India). Isolations were made and F. udum was obtained from
all the locations. All the cultures were purified and single spore isolates were obtained following standard
mycological techniques. All these isolates were tested for virulence using a common susceptible cultivar ICP 2376
following root dip technique. All the virulent cultures were stored at 4 0 C in the laboratory.
Suresh Pande and Mamta Sharma
Milestone: Mapping of one intra-specific RIL population for dry root resistance conduced (PMG/SP) 2008
Mapping of RILs populations to DRR resistance under controlled environment: One hundred and twenty-six
RILs and parents (JG 62 and ICCV 2) were evaluated for DRR resistance using paper towel technique under
controlled environment conditions. Each entry was replicated thrice. Of the 126 RILs evaluated, ICCX 930111- 57-
1-1-1-1SP-1-1-1-BP-1BP showed a resistant reaction (3 rating on 1 - 9 scale), while 15 entries were moderately
resistant (3.1 to 5 rating). Both parents showed a susceptible reaction.
Suresh Pande/PM Gaur
Activity 6A.3.1: Advanced generation inter-specific derivatives with resistance to Helicoverpa and BGM
generated using wild Cicer from different gene pools
Milestone: Strategies to cross wild Cicer between primary, secondary and tertiary gene pools developed (NM) 2008
In collaboration with Washington State University, Pullman, USA crossing program was initiated to cross perennial
Cicer with annual as well as cultivated Cicer. Pollen germination was observed between intra-perennial Cicer
species. It is possible to have successful pollination and fertilization between annual species C. reticulatum and
perennial species C. oxyodon and C nurstichacum, as well as between cultivated chickpea and C. oxyodon and C.
nursticum, but mature seeds were not observed. Since perennial Cicer does not set flowers at ICRISAT Patancheru,
techniques were developed to preserve pollen of perennial Cicer for use at ICRISAT at a later date. Germination
medium for annual and perennial Cicer pollen has also been developed.
Nalini Mallikarjuna and WSU partners
Milestone: Fifteen stable inter-specific derivatives using resistant wild Cicer from secondary gene pool generated
and screened for Helicoverpa, AB, BGM, and good agronomic characters under field conditions
(NM/HCS/SP/PMG) 2010
Cicer accessions ICC 17159, IG 73074, IG 72937, IG 72933, and IG 72934, which have shown resistance to
Botrytis grey mold were used in the crossing program. Seed set varied depending upon the pollen parent used in the
crossing program. The F 2 progenies from the crosses involving wild Cicer species IG 73074 and ICC 17159 are
listed in Table 3. Chi square analysis of the progenies showed that the ratio of resistant to susceptible plants was 1:
3, and hence BGM resistance introgressed from wild Cicer was monogenic and recessive.
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