ICRISAT Archival Report 2006 - The seedlings of success in the ...

Milestone C.3.1.8: Four hundred wild and cultivated Sorghum accessions from Kenya genotyped for 30 SSR
markers (DK, SdV, FS + NARS, 2007)
Milestone C.3.1.9: Paper accepted on the in situ diversity of 472 Sorghum varieties collected in 79 villages in
Niger (FS, BG, JN + NARS + CIRAD + IRD, 2007)
Milestone C.3.1.10: Comparative phylogeography of wild, weedy and cultivated Sorghums in Mali published
(FS, PST, NARS + CIRAD, 2008)
Milestone C.3.1.11: Comparative phylogeography of wild, weedy and cultivated Sorghums in Kenya published
(FS, SdV, DK, NARS + University of Free State, 2008)
Milestone C.3.1.12: Diversity assessment of sorghum and chickpea published
(HDU/CTH/RKV/CLLG/SS/DH/SC/JB, 2009)
A journal article “Development of a composite collection for mining germplasm possessing allelic variation for
beneficial traits in chickpea’ published in Plant Genet. Resources 4: 13-19.
A poster “Genotypic and phenotypic variation in the global collection of chickpea (Cicer aritienum L.)”
presented at the Third International Conference on Legume Genomics and Genetics, 9-13 April 2006, Brisbane,
Queensland, Australia.
HD Upadhyaya, SL Dwivedi, CLL Gowda, RK Varshney,
DA Hoisington,and S Chandra
Milestone C.3.1.13: Data sets for sorghum and chickpea composite set made available globally via Internet
(JB/HDU/CTH/RKV/CLLG/SS/DH/SC, 2008)
Milestone C.3.1.14: Diversity and population structure of groundnut composite collection analyzed and
reference set (300 accessions) established (HDU/RB/RKV/CLLG/DH/JB, 2008)
A preliminary analysis of data on 916 groundnut accessions and 21 SSR markers was carried out using DARwin
5.0 Structure program to determine the population structure of the composite collection. The software removes
all those accessions/markers that have high missing values and finally 900 accessions were considered for
principal coordinate analysis considering the taxonomical classification of Arachis, i.e. at the level of two
subspecies and six botanical varieties. The analysis detected a total of 506 alleles, ranging from 6 (7H6) to 47
(5D5) with a mean of 24.1 alleles per locus and mean PIC value of 0.797 (ranging from 0.483 to 0.923)
fastigiata: Red; hypogaea: Black; Wild: Light Green
Fig. 3. Tree diagram of 900 accessions with 21 SSR markers at subspecies level.
Principal coordinate analysis (PCoA) using DARwin 5.0 on subspecies revealed that both hypogaea and
fastigiata formed distinct clusters however, a number of fastigiata accessions also grouped with hypogaea types
63