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ICRISAT Archival Report 2006 - The seedlings of success in the ...

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Milestone: At least 8 promis<strong>in</strong>g transgenic events <strong>of</strong> groundnut conta<strong>in</strong><strong>in</strong>g DREB1A gene identified and <strong>the</strong>ir<br />

drought tolerance characterized under conta<strong>in</strong>ed field conditions (KKS/VV/RA) 2008<br />

Milestone: Three promis<strong>in</strong>g transgenic events <strong>of</strong> groundnut identified for drought tolerance and characterized under<br />

conta<strong>in</strong>ed field conditions (KKS/VV/RA) 2009<br />

Milestone: One or two transgenic events <strong>of</strong> groundnut used for <strong>in</strong>trogression <strong>in</strong>to locally adapted genotypes with<br />

better adaptation and <strong>the</strong> progeny characterized and evaluated (KKS/VV/SNN/RA) 2010<br />

Milestone: 15 - 20 <strong>in</strong>trogressed transgenic l<strong>in</strong>es <strong>of</strong> groundnut with improved tolerance to water-limit<strong>in</strong>g conditions<br />

evaluated and development <strong>of</strong> commercialization package <strong>in</strong>itiated (KKS/VV/SNN/RA) 2011<br />

Activity 6B.2.2: Mapp<strong>in</strong>g and marker-assisted breed<strong>in</strong>g for sal<strong>in</strong>ity tolerance <strong>in</strong> groundnut<br />

Milestone: At least 10 genotypes with superior sal<strong>in</strong>ity tolerance identified (VV/RA/SNN/RKV) 2007<br />

Screen<strong>in</strong>g <strong>of</strong> 288 groundnut genotypes has been carried out for sal<strong>in</strong>ity tolerance. Over 6-fold range <strong>of</strong> variation for<br />

sal<strong>in</strong>ity tolerance was observed <strong>in</strong> this material. High yield<strong>in</strong>g varieties (ICGV 87187, ICGV 86155, ICGV 00309,<br />

ICGV 93382, ICGV 97245, ICG 3027, ICG 76, ICG 5195, ICG 6892, and ICG 11651) would be used to screen <strong>the</strong><br />

molecular diversity and most diverse parental genotype comb<strong>in</strong>ations will be used to develop mapp<strong>in</strong>g populations.<br />

Confirmation <strong>of</strong> <strong>the</strong> tolerance is under way.<br />

V<strong>in</strong>cent Vadez<br />

Milestone: Mapp<strong>in</strong>g populations between contrast<strong>in</strong>g parents for sal<strong>in</strong>ity tolerance developed to identify QTLs<br />

(VV/RA/SNN/RKV) 2008<br />

A set <strong>of</strong> contrast<strong>in</strong>g parents for sal<strong>in</strong>ity tolerance based on <strong>the</strong> screen<strong>in</strong>g for pod yield under sal<strong>in</strong>ity has been given<br />

to <strong>the</strong> breed<strong>in</strong>g group. Contrast<strong>in</strong>g parents will be also assessed for <strong>the</strong>ir level <strong>of</strong> polymorphism, us<strong>in</strong>g a number <strong>of</strong><br />

SSR markers. After confirmation <strong>of</strong> <strong>the</strong> contrast <strong>in</strong> sal<strong>in</strong>ity tolerance between <strong>the</strong> parents, and after ensur<strong>in</strong>g that<br />

parents have sufficient level <strong>of</strong> polymorphism at <strong>the</strong> DNA level, <strong>the</strong> F 2 populations will be advanced by s<strong>in</strong>gle-seed<br />

descent.<br />

V<strong>in</strong>cent Vadez and R Aruna<br />

Milestones: QTLs for sal<strong>in</strong>ity tolerance identified (VV/SNN/RA) 2011<br />

Tolerant and sensitive groundnut genotypes for sal<strong>in</strong>ity tolerance have been dentified and crosses are currently be<strong>in</strong>g<br />

made.<br />

V Vadez, SN Nigam and Aruna R<br />

Output B. Annually knowledge <strong>of</strong> <strong>the</strong> improvements <strong>of</strong> <strong>the</strong> biotechnological and conventional tools designed<br />

to facilitate drought and sal<strong>in</strong>ity tolerance breed<strong>in</strong>g and germplasm <strong>of</strong> mandate crops and associated capacity<br />

build<strong>in</strong>g made available to partners <strong>in</strong>ternationally<br />

Chickpea<br />

MTP Output Target 200: New Kabuli and desi germplasm with early maturity to avoid drought developed and<br />

made available to partners<br />

Output target 6B.1: Identification <strong>of</strong> QTLs for drought avoidance root traits and sal<strong>in</strong>ity tolerance <strong>in</strong><br />

chickpea<br />

Activity 6B.1.1: Mapp<strong>in</strong>g and marker-assisted breed<strong>in</strong>g for drought tolerance <strong>in</strong> chickpea<br />

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