ICRISAT Archival Report 2006 - The seedlings of success in the ...

generates mostly single nucleotide polymorphisms or SNPs (in genes) and can be controlled to produce a high
density of point mutations causing a variety of lesions including nonsense and missense mutations. After
treating the seeds of the selected pearl millet inbred line with several concentrations (5 mM, 10 mM, 15 mM, 20
mM, 25 mM, 30 mM, 35 mM, 40 mM, 45 mM, 50 mM, 55 mM and 60mM), recommended in literature our
results suggested that all the mutagen concentrations above 5 mM are lethal to the pearl millet genome.
Subsequently a lower range of concentration of mutagen (1 mM, 2 mM, 3 mM, 4 mM, 5 mM, 6 mM, 7 mM, 8
mM, 9 mM, and 10 mM) for different treatment time (4 hrs, 8 hrs, 12 hrs) was used. As a result, finally about
2000 seeds each were treated with three mutagen concentrations i.e. 5 mM, 9 mM and 10 mM (each with 4 hrs)
that should provide 60% 50% and 40% plants survived. The treated seeds were sown in 12” diameter pots and
after about two weeks time, these plants were transplanted into field. In fields all plants were selfed and finally a
total of 2,581 M1 lines have been harvested. These include 1169 lines from 5 mM, 737 from 9 mM and 675
from 10 mM. These lines will be advanced to M2 generation and another set of M1 lines will be generated.
RK Varshney, T Mahender, CT Hash and DA Hoisington
Activity D.1.2. Develop suitable contrasting parental lines for salinity tolerance in staple crops
Milestone D.1.2.1: Suitable contrasting parental lines for salinity tolerance in chickpea for the development of
RILs provided (VV/RKV/HDU/PMG, 2007)
A set of contrasting genotypes for salinity tolerance identified and three crosses have been performed:
ICC 6263 (sensitive, DF 70) x ICC 1431 (tolerant, DF 69)
ICC 15802 (sensitive, DF 66) x ICC 9942 (tolerant, DF 63)
ICCV 2 (sensitive, DF 39) x JG 11 (tolerant, DF 40)
Parents used for these 3 crosses have been chosen based on the similarity in phenology (DF = days to
flowering), since it was found that the number of days to flowering has some influence on the level of salinity
tolerance in the conditions where we assess the materials. Diversity analysis using SSR markers is in progress.
V Vadez, RK Varshney, HD Upadhyaya and PM Gaur
Milestone D.1.2.2: Suitable contrasting parental lines for salinity tolerance in groundnut for the development of
RILs provided (VV/SNN/AR/RKV/HDU, 2008)
A group of genotypes showing good contrast for salinity tolerance has been identified for use in groundnut
breeding. The choice of tolerant and sensitive materials has been based on a high and low pod yield under saline
conditions, with all genotypes obtaining a good yield under control conditions.
V Vadez, SN Nigam, R.Aruna, RK Varshney and HD Upadhyaya
Activity D.1.3: Assemble and make available for distribution the existent RILs of staple crops and small
millets that are in the public domain in seed and DNA form
Milestone D.1.3.1: Seed multiplied for RIL populations for different crops
(HDU/DH/CLLG/CTH/RKV/SS/RB/SNN/AR/PMG/KPS/KNR/BVSR, and others, 2009)
Milestone D.1.3.2: DNA of different RIL populations isolated
(HDU/DH/CLLG/CTH/RKV/SS/RB/SNN/AR/PMG/KPS/KNR/BVSR, and others, 2010)
Milestone D.1.3.3: Marker and phenotype databases for the available RIL mapping populations curated
(HDU/JB/DH/CTH/CLLG/RKV/SS/RB/SNN/AR/PMG/KPS/KNR/ BVSR, and others, 2010)
Output target D.2: DNA extracts of sub sets of germplasm conserved for utilization (2011)
Activity D.2.1: Conserve DNA extracts of sub sets of germplasm for utilization
Milestone D.2.1.1: DNA extracts of mini core and reference sets of chickpea conserved (HDU/RKV/DH/CLLG,
2008)
DNA was extracted from the chickpea reference collection consisting of the mini core collection.
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