ICRISAT Archival Report 2006 - The seedlings of success in the ...

Activity C.11.3: Comparative genomics tools to aid marker development. (JB/SS/CTH/RKV and others)
Milestone C. 11.3.1: Development of appropriate software pipelines for mining of markers from public data
(2008, Annual)
Pipelines for the SNP, from public EST data are now available on HPC (http://hpc.icrisat.cgiar.org/pbsweb).
Output D: RILs of staple crops and small millets developed/assembled and DNA extracts conserved and
distributed
MTP Output Targets 2006
Genetic diversity of chickpea composite collection determined
RILs of groundnut and chickpea (WUE, diseases) assembled
Output target D.1: RILs of staple crops assembled (2009)
Activity D.1.1: Assemble RILs of staple crops
Milestone D.1.1.1: RILs of chickpea (root traits, resistance to Helicoverpa, fusarium wilt, BGM, and salinity
tolerance) assembled (PMG/HDU/CLLG, 2006)
ICRISAT has developed and is maintaining three RIL populations (Annigeri × ICC 4958, ICCV 4958 × ICC
1882, ICCV 283 × ICC 8261 ) for root traits, two for fusarium wilt resistance (ICCV 2 × JG 62, WR 315 × C
104) and one for Helicoverpa resistance (ICC 505 EB x Vijay). We assembled three RIL populations for root
traits and fusarium wilt resistance from other institutes during 2006. These include two intraspecicfic RIL
populations, JG 62 x ICC 4958 (fusarium wilt resistance and root traits) and Vijay x ICC 4958 (root traits) from
National Chemical Laboratory, Pune, India and one interspecific RIL population, Cicer arietinum (ICC 4958) x
C. reticulatum (PI 489777) (root traits) from Washington State University, Pullman, USA. The seed of these
RILs are being multiplied during the 2006/07 crop season and would be submitted to the genebank.
Milestone D.1.1.2: RILs of groundnut (WUE - 4, rust – 2, and LLS – 2) assembled (SNN/HDU, 2006)
We developed four RIL populations, two each for late leaf spot (ICGV 99001 x TMV 2 and ICGV 99004 x
TMV2) and rust resistance (ICGV 99003 x TMV 2 and ICGV 99005 x TMV2 ). TMV 2 was used as susceptible
parent for both rust and late leaf spots in all four populations. The four resistant parents were interspecific
derivatives involving cultivated Arachis hypogaea (AABB genome, 2 n = 4 x = 40) and three wild Arachis
species, A. villosa, A. cardenasii, and A. stenosperma (all A genome 2 n = 2 x =20). ICGV 99001 is an
interspecific derivates from a cross between cultivated Robut 33-1 (subsp. hypogaea var. hypogaea) and Arachis
villosa. ICGV 99003, ICGV 99004, and ICGV 99005 are interspecific derivates from three-way crosses
between hybrids of two cultivated lines and wild species. ICGV 99003 was developed from crosses involving a
hybrid of cultivated types with A. stenosperma. ICGV 99004 and ICGV 99005 were developed from a three way
cross between hybrids of two cultivated types with A. cardenasii. The seeds of all four RIL populations are
conserved in the genebank.
Milestone D.1.1.3: RILs of pigeonpea (one population of wilt resistance) assembled (KBS/HDU, 2008)
Milestone D.1.1.4: RILs of pearl millet (3 populations) assembled (CTH/HDU, 2008)
Milestone D.1.1.5: RILs of sorghum (grain mold – 2, stem borer –3, and shoot fly -2) assembled (BVSR/HCS,
2008)
Milestone D.1.1.6: TILLING population of pearl millet developed (RKV/CTH/DH, 2009)
To generate the TILLING population in pearl millet, the inbred line “P1449-2-P1” has been chosen, as it is one
of the parental genotype of a mapping population (PT 732B x P1449-2-P1) developed and maintained at
ICRISAT and segregates for plant height (d2), downy mildew resistance and stover grain quality. It is the tall
parent, witch serves as a good source of downy mildew resistance for the improvement of local cultivars. The
chemical mutagen ethyl methane sulfonate (EMS) was selected to develop the TILLING population, as it
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