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ICRISAT Archival Report 2006 - The seedlings of success in the ...

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functionality and user <strong>in</strong>terfaces cont<strong>in</strong>ue to be modified to suit user requirements. <strong>The</strong> LIMS application has<br />

also been extended and adapted for use at <strong>ICRISAT</strong>/IITA/ILRI - Nairobi and IITA-Ibadan. A workshop was<br />

conducted at <strong>ICRISAT</strong>-Nairobi to demonstrate <strong>the</strong> s<strong>of</strong>tware to potential users and obta<strong>in</strong> feedback. <strong>The</strong> s<strong>of</strong>tware<br />

has also been shared with several universities and private partners under <strong>the</strong> terms <strong>of</strong> <strong>the</strong> GNU general public<br />

license.<br />

B Jayashree, DA Hois<strong>in</strong>gton, S Senthilvel, RK Varshney,<br />

DKiambi and S de Villiers<br />

Activity C.10.2: Integrated database development with web <strong>in</strong>terfaces and <strong>in</strong>teroperability requirements.<br />

Milestone C.10.2.1: Development <strong>of</strong> database and middleware with GUI (JB/DH/ and o<strong>the</strong>rs, 2007)<br />

<strong>The</strong> LIMS –ICRIS adaptor allows <strong>the</strong> flow <strong>of</strong> genotyp<strong>in</strong>g data from <strong>the</strong> LIMS application <strong>in</strong>to <strong>the</strong> ICRIS<br />

database. <strong>The</strong> database is expected to <strong>in</strong>tegrate genotyp<strong>in</strong>g <strong>in</strong>formation with genetic resource and phenotype<br />

<strong>in</strong>formation. Progress was made this year <strong>in</strong> cod<strong>in</strong>g for generic middleware to this database to br<strong>in</strong>g it <strong>in</strong><br />

compliance with <strong>the</strong> GCP(generation challenge program) platform. This will allow for <strong>the</strong> database to become<br />

<strong>in</strong>teroperable with o<strong>the</strong>r databases and repositories available <strong>in</strong> <strong>the</strong> public doma<strong>in</strong>. A GCP platform compliant<br />

data source API (application program <strong>in</strong>terface) is also be<strong>in</strong>g developed that will allow data consumers such as<br />

publicly available analytical or visualization tools to access data with<strong>in</strong> <strong>the</strong> database.<br />

B Jayashree and DA Hois<strong>in</strong>gton<br />

Milestone C.10.2.2: Alpha and beta test<strong>in</strong>g <strong>of</strong> database by users (CTH/SS/RKV/HDU/RB/VV/and o<strong>the</strong>rs)<br />

Milestone C.10.2.3: Curation <strong>of</strong> data with <strong>in</strong>volvement <strong>of</strong> data providers.<br />

Output target C.11: Development <strong>of</strong> data analysis tools<br />

Activity C.11.1: iMAS a decision support system for marker aided breed<strong>in</strong>g.<br />

Milestone C.11.1.1: Fur<strong>the</strong>r development and test<strong>in</strong>g <strong>of</strong> application (SC/DH/JB, 2007).<br />

<strong>The</strong> goal <strong>of</strong> this two-year project (2005-06) was to develop an <strong>in</strong>tegrated decision support system, called iMAS,<br />

to seamlessly facilitate marker-assisted plant breed<strong>in</strong>g by <strong>in</strong>tegrat<strong>in</strong>g freely available quality s<strong>of</strong>tware <strong>in</strong>volved<br />

<strong>in</strong> <strong>the</strong> journey from phenotyp<strong>in</strong>g-and-genotyp<strong>in</strong>g <strong>of</strong> genetic entities to <strong>the</strong> identification and application <strong>of</strong> traitl<strong>in</strong>ked<br />

markers, and provid<strong>in</strong>g simple-to-understand-and-use onl<strong>in</strong>e decision guidel<strong>in</strong>es to correctly use <strong>the</strong>se<br />

s<strong>of</strong>tware, <strong>in</strong>terpret and use <strong>the</strong>ir outputs. <strong>The</strong> project was structured <strong>in</strong>to n<strong>in</strong>e activities, namely: A1: Analyze<br />

potentially useful free s<strong>of</strong>tware, A2: Select s<strong>of</strong>tware for <strong>in</strong>clusion <strong>in</strong> iMAS, A3: Develop iMAS system, A4:<br />

Develop & <strong>in</strong>corporate onl<strong>in</strong>e decision guidel<strong>in</strong>es, A5: Test iMAS system, A6: Ref<strong>in</strong>e iMAS system, A7:<br />

Develop iMAS user manual/tutorial, A8: Release <strong>of</strong> and Tra<strong>in</strong><strong>in</strong>g <strong>in</strong> iMAS, A9: Consultation and support.<br />

Five different s<strong>of</strong>tware have been <strong>in</strong>tegrated <strong>in</strong> this pipel<strong>in</strong>e along with onl<strong>in</strong>e decision support and <strong>the</strong><br />

<strong>in</strong>tegration <strong>of</strong> two more s<strong>of</strong>tware is <strong>in</strong> progress <strong>in</strong> <strong>2006</strong>. Integrated s<strong>of</strong>tware <strong>in</strong>clude IRRISTAT, Gmendel,<br />

PlabQTL, POPMIN and GGT while <strong>the</strong> <strong>in</strong>tegration <strong>of</strong> W<strong>in</strong>QTLCartographer and TASSEL is <strong>in</strong> progress. <strong>The</strong><br />

pipel<strong>in</strong>e has been modified to <strong>in</strong>corporate user comments, two major workshops were conducted<br />

accommodat<strong>in</strong>g over 40 scientists from <strong>the</strong> NARS besides <strong>ICRISAT</strong> staff and <strong>in</strong>terested private partners to test<br />

<strong>the</strong> application. Besides, project partners reviewed <strong>the</strong> application and <strong>the</strong>ir suggestions are be<strong>in</strong>g <strong>in</strong>corporated.<br />

S Chandra, DA Hois<strong>in</strong>gton and B Jayashree<br />

Activity C.11.2: High performance comput<strong>in</strong>g toolbox.<br />

Milestone C.11.2.1: Extension <strong>of</strong> exist<strong>in</strong>g tools with user friendly <strong>in</strong>terfaces (JB/RKV/DH/SC and o<strong>the</strong>rs).<br />

<strong>The</strong> pipel<strong>in</strong>es and standalone s<strong>of</strong>tware available with<strong>in</strong> <strong>the</strong> comparative genomics and population genetics<br />

toolboxes on <strong>the</strong> high performance computer have been extended through <strong>2006</strong>. <strong>The</strong> comparative genomics<br />

toolbox now <strong>in</strong>cludes a suite <strong>of</strong> parallelized programs for marker m<strong>in</strong><strong>in</strong>g and detection from large public<br />

datasets and open source s<strong>of</strong>tware for comparative sequence analysis and phylogeny. <strong>The</strong> population genetics<br />

toolkit <strong>in</strong>cludes a parallelized version <strong>of</strong> <strong>the</strong> program ‘structure’ with user <strong>in</strong>terfaces and visualization s<strong>of</strong>tware<br />

along with format conversion tools for a variety <strong>of</strong> popularly used analysis s<strong>of</strong>tware.<br />

B Jayashree, RK Varshney, DA Hois<strong>in</strong>gton, AG Sylvester,<br />

S Chandra, MS Hanspal,and VT Jagdesh<br />

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