ICRISAT Archival Report 2006 - The seedlings of success in the ...

IPC 804 x 81B ) and two tester lines (Tift 383 and Tift 186). Overall a total of 336 markers displayed
polymorphism in at least one of 11 mapping populations. The polymorphic markers in different mapping
populations ranged from 113 (Tift 23DB-1-P5 x WSIL-P8) to 151 (ICMB 841-P2 x 863-P3) with an average of
115 markers per population. Two mapping populations (i.e. ICMB 841-P2 x 863-P3 and 81B-P6 x ICMP 451-
P8) are being advanced to RILs (at present F6 lines) and therefore in the first instance, these mapping
populations will be genotyped with the polymorphic markers.
RK Varshney, T Mahender, CT Hash, S Senthilvel and DA Hoisington
Output target D.5: Agriculturally beneficial microorganisms assembled for utilization (2010)
Activity D.5.1: Assemble and conserve agriculturally beneficial microorganisms for utilization and
distribution
Milestone D.5.1.1: Agriculturally beneficial microorganisms from diverse environments accessed and
characterized for 6 different traits – P- solubilization, antagonism to disease-causing fungi, pathogenicity to
insect-pest, siderophore production (OPR, Annual)
A total of 523 isolates involving bacteria, fungi, and actinomycetes were added to the short to medium term
storage, for further studies. All these were picked to represent diversity in a soil sample from a given cropproduction
system and had at least one agriculturally beneficial trait such as ability to solubilize rock phosphate,
plant growth promotion, antagonism to plant pathogenic fungi, pathogenicity to insect-pests. Most of these were
bacteria and were characterized further for confirmation of the traits they were picked for and to learn those with
more than one beneficial traits. The traits studied were P-solubilization, antagonism to Macrophomina
phaseolina (a soil-borne root rot fungus), siderophore production (indicator of plant growth promotion) and
presence crystalline protein. Eight were positive for at least two traits and four (SRI77, EB24, SB26, HIB67)
had crystal proteins of the type found in Bacillus thuringiencis (Table 8).
Table 8. Characterization of bacterial isolates for multiple beneficial traits
S. No Isolate
number
P-solubilization Antagonistic to
M. phaseolina
Siderphore
production
Crystalline
protein
1 SRI 77 - - - +
2 SRI 151 + + + -
3 SRI 156 + + + -
4 SRI 158 + + + -
5 SRI 178 + + + -
6 SRI 229 + + + -
7 SRI 305 + + + -
8 EB24 - + ND +
9 SB26 + + ND +
10 HIB67 - - ND +
ND = Not Determined
OP Rupela
Milestone D.5.1.2: Existing collection of agriculturally beneficial microorganisms conserved for medium and
long-term storage system and annually 20% germplasm attended (OPR, Annual)
No long-term storage could be undertaken due to malfunctioning of the Freeze-drier, an equipment needed for
the purpose.
OP Rupela
Milestone D.5.1.3: Requested agriculturally beneficial microorganisms distributed to bonafide users for
utilization (OPR, Annual)
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