ICRISAT Archival Report 2006 - The seedlings of success in the ...

project planning meeting was held in April 2006 in Nairobi, followed by a phenotyping workshop. Project
partners agreed to work on a regional composite of 1720 accessions representing landraces, farmer varieties and
breeders’ lines. Seed multiplication and phenotyping of 1260 Sorghum accessions has started in Eritrea, Kenya,
Uganda, Tanzania and Sudan. 27 consensus descriptors representing all the sorghum developmental stages are
being used for morphological characterization. These are partially derived from the IPGRI descriptor lists for
Sorghum. Characterization data is being generated and documented in the Access Database developed during
the phenotyping workshop. Genotyping activities have been initiated, starting with the extraction, quantification
and quality checks of DNA from 196 sorghum samples from Tanzania. The DNA will be used both for
genotyping and repository as part of the GCP Sorghum global collection. Initial genotyping has started with the
screening of 196 accessions using 6 polymorphic SSR markers. All the 24 SSR markers that will be used for
genotyping have been optimized and screened for polymorphism. A database with an initial entry of 1260
germplasm accessions that are being phenotyped and genotyped by the participating NARS has been developed.
The information on the accessions mainly comprises passport data and will be used to identify duplicates
through the unique identifiers. Two PhD students have been registered at the University of Wad Medani and
University of Free State, South Africa to phenotype and genotype 400 samples from Sudan. 4 MSc students
have been registered in national universities in Ethiopia, Kenya, Uganda and Tanzania to advance the
phenotyping and genotyping activities.
D Kiambi, DA Hoisington, CT Hash and S de Villiers
Milestone C.3.3.3: Molecular characterization of germplasm held by ESA NARS completed (DK, 2007)
Milestone C.3.3.4: Phenotypic and molecular data for sorghum standardized and analyzed (DK, 2008)
Activity C.3.4: Development of a database for documentation and retrieval of morphological and
molecular data
Milestone C.3.4.1: Database of passport information, farmer-knowledge, pedigrees, phenotyping and
genotyping data of sorghum accessions held in ESA national genebanks and international nurseries developed
(DK, 2008)
Output target C.4: Core, mini core, and or reference sets of germplasm evaluated for utilization in Asia
(2009)
Activity C.4.1: Evaluate core/mini core/reference sets of staple crops and small millets for agronomic
traits
Milestone C.4.1.1: Mini core collections of chickpea, groundnut, and pigeonpea evaluated in multilocations in
Asia (HDU/CLLG/NARS, 2008)
Chickpea: 211 accessions of chickpea mini core collection with five control cultivars (Annigeri, ICCV 2, ICCV
10, L 550, KAK 2, G 130) were evaluated in augmented designed trials at Patancheru, Palampur, Chandigarh,
and Bangalore in India, and at Culiacan in Mexico. Data is awaited for out stations. At ICRISAT, Patancheru
ICCs 708, 3631, 6816, 8950, and 16903 (2.9-3.3 t ha -1 ) were top five accessions with greater seed yield than all
the five control cultivars (1.2-2.8 t ha -1 ). ICC 16309 (42 days to 50% flowering and 105 days to maturity) was
early flowering and early maturing than high yielding controls ICCV 10 (45 and 110 days ) and Annigeri (45
and 108 days)
Pigeonpea: Pigeonpea mini core collection consisting of 146 accessions was evaluated with four control
cultivars (ICP 11543, ICP 6971, ICP 8863, ICP 7221) in augmented designed trials at Patancheru, Dholi,
Bangalore, Kanpur, Khaegone, Sardar Krishinagar, and Badnapur in India, and at Dubai in UAE. Data is
awaited for out stations. At ICRISAT, Patancheru, ICPs 3451, 4167, 6123, 8255, and 14722 (2.6 – 2.8 t ha -1 )
produced greater seed yield than the best control cultivar ICP 8863 (2.0 t ha -1 ).
Groundnut: 184 accessions of groundnut mini core collection were evaluated with four control cultivars
(Gangapuri, M 13, ICGS 44, ICGS 76) in augmented designed trials at Patancheru, Jalgaon, Raichur,
Durgapura, and Aliyanagar in India, Qingdao in China, and two sets at Lilongwe in Malawi. Data is awaited for
out stations. At ICRISAT, Patancheru ICGs 3992, 10185, 12625, and 14482 (4.3 – 4.6 t ha -1 ) had greater pod
yield than the high yielding control cultivar M 13 (4.2 t ha -1 ). ICGs 115, 397, 3746, 10890, and 15042 (>75%)
had greater shelling turn over, and ICGs 4746, 5662, 6993, and 9905 had greater seed size (100-110 g).
HD Upadhyaya and CLL Gowda
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