ICRISAT Archival Report 2006 - The seedlings of success in the ...

Progress reported towards the achievement of milestone for 2007 will contribute towards achievement of the
milestones listed below.
Milestone: At least 8 promising transgenic events of groundnut containing maize psy1 gene identified and their
stability characterized under contained greenhouse conditions (KKS/SNN) 2008
Milestone: Three promising transgenic events of groundnut identified for high ß-carotene production and
characterized under contained field conditions (KKS/SNN) 2009
Milestone: One or two transgenic events of groundnut with high beta-carotene content used for introgression into
locally adapted genotypes and the progeny characterized and evaluated (KKS/SNN/RA) 2010
Milestone: 5 - 7 introgressed transgenic lines of groundnut with improved beta-carotene content evaluated and
development of commercialization package initiated (KKS/SNN/RA) 2011
Activity 6C1.2: Develop pigeonpea transgenic events for enhanced production of beta-carotene
Milestone: 50 transgenic events of pigeonpea with maize psy1 gene developed and screened for high ß-carotene
production in the contained greenhouse (KKS/KBS/KML) 2007
Development of pigeonpea transgenic events for enhanced level of ß-carotene: Agrobacterium-mediated genetic
transformation is being carried out regularly using the binary vectors containing maize psy1 gene driven by oleosin
promoter for generating pigeonpea transgenic events with enhanced level of ß-carotene. The putative transgenic
pigeonpea shoots obtained under antibiotic selection pressure are being elongated for rooting. About 40 putative
transgenic plants with maize psy1 have been transferred to the containment greenhouse for further analysis. The
primers specific to the coding sequence of psy1 genes were designed and conditions for PCR amplification were
optimized. The primary T 0 putative pigeonpea plants are being molecularly analyzed using PCR for the presence of
psy1 gene.
Development of pigeonpea transgenic events for enhanced level of methionine: In separate studies, binary gene
constructs containing SSA gene driven by vicillin promoter (pHS723:SSA) in Agrobacterium tumefaciens strain C
58 is being used for the development of pigeonpea transgenics for enhanced level of seed methionine content.
Around 20 putative transgenic plants were transferred to the containment greenhouse for further analysis. The
primers specific to the coding sequence of SSA genes were designed and conditions for PCR amplification were
optimized. The primary putative transgenic pigeonpea plants obtained under antibiotic selection pressure are being
analyzed using PCR for the presence of SSA gene.
A Vanamala, KK Sharma and KB Saxena
Progress reported towards the achievement of milestone for 2007 will contribute towards achievement of the
milestones listed below.
Milestone: At least 8 promising transgenic events of pigeonpea containing maize psy1 gene identified and their
stability characterized under contained greenhouse conditions (KKS/KBS/KML) 2008
Milestone: Three promising transgenic events of pigeonpea identified for high ß-carotene production and
characterized under contained field conditions (KKS/KBS/KML) 2009
Milestone: One or two transgenic events of pigeonpea with high beta-carotene content used for introgression into
locally adapted genotypes and the progeny characterized and evaluated (KKS/KML) 2010
Milestone: 5 - 7 introgressed transgenic lines of pigeonpea with enhanced beta-carotene content evaluated and
development of commercialization package initiated (KKS/KML) 2011
MTP Output Target 2006: 10 new groundnut varieties with resistance to aflatoxin contamination made available to
partners
Output target 6C.2: Transgenic groundnut with enhanced resistance to Aspergillus flavus and aflatoxin
production identified and available for introgression into regionally adapted germplasm
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