ICRISAT Archival Report 2006 - The seedlings of success in the ...

The genotyping of reference collections of chickpea accessions will be started with optimized multiplexes by
using Genetic Analyzer (ABI 3100).
HD Upadhyaya, N Lalitha, RK. Varshney, J Kashiwagi,
L Krishnamurthy and S Chandra
Milestone C.5.4.4: Groundnut reference set phenotyped for traits associated with drought resistance
(VV/HDU/RKV, 2009)
Milestone C.5.4.5: Groundnut reference set genotyped with 100 SSR markers (HDU/RKV/DH, 2010)
Milestone C.5.4.6: Reference set of chickpea (300 accessions) utilized for candidate gene diversity for mining
the drought tolerant alleles (RKV/HDU/JK/DH/PMG, 2010)
Under Allelic Diversity on Orthologous Candidate genes (ADOC), identification of drought responsive genes is
underway. Currently, DREB1a and DREB2a genes are being identified in chickpea by using in silico analysis
(see Output Target D.3).
RK Varshney, HD Upadhyaya, B Jayashree and DA Hoisington
Milestone C.5.4.7: Diversity analyzed for the molecular markers and markers associated with root traits
identified (HDU/JK/RKV/LK/SC/NL, 2011)
Milestone C.5.4.8: Diversity analyzed for the molecular markers and markers associated with drought traits
identified (HDU/RKV/DH, 2012)
Output target C.6: Germplasm sets evaluated for utilization in Africa (2009)
Activity C.6.1: Evaluation (field test) of the global pearl millet core set at Sadore, Niger
Milestone C.6.1.1: M Sc thesis on evaluation of the pearl millet core set at Sadore, Niger, completed and data
available in Excel format (BH/HDU, 2007)
The global pearl millet core collection was grown in a replicated trial at the ICRISAT Sahelian Center near
Niamey, Niger, in the Rainy Season 2006. Ms Jenny Coral Padilla, MSc student from University of Hohenheim,
Stuttgart, Germany, was involved in the characterization. Data analysis and MSc thesis write-up are underway.
BIG Haussmann and HD Upadhyaya
Activity C.6.2: Characterize core collection of finger millet and identify materials for regional evaluation
Milestone C.6.2.1: Core collection of finger millet characterized for morpho-agronomic and end use traits
(MAM/SGM/HDU, 2007)
Finger Millet: 506 finger millet germplasm accessions of core collection from India were planted and
characterized at Kiboko (2005/06) and Alupe (April-Sept 2006). Preliminary principal component analysis
results from Kiboko based on 15 quantitative traits showed 57% of the variability in the germplasm accounted
for by the first two principal components (PCs). High positive loadings on PC1 were contributed by days to 50%
flowering, plant heights, leaves per plant, leaf lengths, leaf widths, neck lengths, peduncle length, stem diameter
and high negative loadings from heads per plant, number of nodal tillers, and number of productive tillers.
Though generally 6 cluster groups were observed, 3 distinct clusters were evident with no distinct pattern of
grouping based on country of origin as accessions from different countries were found in almost all clusters.
Further analysis including all traits (both qualitative and quantitative) from Kiboko and Alupe will done and
reported fully in the 2007 archival report
MA Mgonja, E Manyasa, E Muange and HD Upadhyaya
Milestone C.6.2.2: Promising and adaptable materials identified and distributed and evaluated in regional
finger millet trials the ESA (MAM/SGM, 2008)
Activity C.6.3: Characterize a sorghum core collection from five African Bio-fortified Sorghum target for
diversity in micro nutritional traits
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