Dokument 1.pdf - OPUS - Friedrich-Alexander-Universität Erlangen ...

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6. Liste der im Pilot-Screen injizierten Fragmente
Seq. #
Insert
(bp)
RNA
# Gen (NCBI o. GLEAN) Acc. # (NCBI) GLEAN #
bester D.m. Hit
(Acc.#, BlastX) protein e-value
Phänotyp pupale
Injektion (Penetranz)
a21 1400 1 similar to Exportin 1 XM_968740 12162 NP_723391 embargoed 2,00E-11 adult lethal (100 %)
a31 800 2
similar to eukaryotic
translation initiation factor
3, subunit 12 XM_968233 7178 NP_611604 CG10306 1,00E-65 - -
124 550 3 similar to predicted protein XM_962797 3920 NP_649293 CG12975 5,00E-22 oogenesis
a15 1350 5
113 800 6
similar to AGAP005116-
PA (coiled-coiled domain
containing?) XM_970351 8842 NP_726051 CG30390 5,00E-83 - -
similar to kynurenine
aminotransferase XM_961781 11119 (w. gap) NP_650121
CG6950,
isoform B
1,00E-
106 - -
29 800 7 GLEAN_08250 - 8250 NP_608350 CG12531 1,10 - -
a16 550 8 similar to rac XM_962956 7747 (w. gap) NP_647718 CG15877 2,00E-10
33 630 9
gene without clear
homologs
no NCBI
annotation no GLEAN
early embryonic
lethal/not fertilized
(100 %)
none, best
BLAST-X hit:
XP_001983639 3,60 legs, head (30/90 %)
Phänotyp larvale
Inj.(Penetranz) Beschreibung
larval lethal (100
%)
pupal appendages
(wings; 80 %)
pupal appendages
(wings; 80 %)
stink glands (40
%)
106 850 10 similar to calcineurin B XM_970177 7534 NP_524874 calcineurin B2 1,00E-88 - adult lethal (66 %)
a30 850 11 similar to translin
no NCBI
annotation 9885 NP_610591 translin 8,00E-66 segmentation (30 %) -
eggs accumulating in
ovarioles/elytra membranous?
membranous deleted?,
antennae shortened, legs sticky
outer rim of elytra slightly bent
upwards
several show one or more legs
or/and antennae tilted inside
the body; most of them and
some others show diff.
misshapings of the hindgut;
alterations of gena triplet: closer
to each other and closer to the
vertex triplet, the bell row
seems to be reduced/prothoracic
stink glands
seem melanotic, but in one it
was gone after co2 anaesth
and pressure; both SPM had no
offspring
anterior defects, ranging from
deletion of some thoracic or
head segments to deletion of
head and thorax and "cuticle
balls",; not dev is increased
Anhang