[Abstract Title]. - Society for Neuroscience

1 Dept Hyg., Kanazawa Univ. Sch. Med., Kanazawa 920-8640, Japan; 2 Kawasaki Med. Univ.,
Kurashiki, Japan; 3 Jikei Med. Col., Tokyo, Japan
Abstract: Lateral line nerve of teleosts locating in the lateral line system is a sensor of current
flow and supposed to be correspondent to deep sensory nerve of mammalian. In order to sense
very minute flow, the wall of lateral line tube should be maintained very thin, whereas the tube
itself becomes bigger according to scale growth. Thus, its morphology seems to be strictly
regulated. When subtraction cloning was subjected to RNAs between scale - gill (SG) in order to
explore mRNA species enriched in the scale, the clones highly homologous to SPARC,
baculoviral IAP repeat containing 2 (birc2), and BCl2-related ovarian killer a (boka) were
isolated. In situ hybridization confirmed birc2 and boca hybridization in flat cells along the ridge.
Hybridization for birc2 was also observed in cells between the ridges. SPARC hybridization was
apparent in flat and small cells between the ridges. birc2 and boka were isolated as mRNAs
enriched in the normal scale but they also abundantly expressed in the cells along the lateral line
tube. Although all the cells in the scale were designated as scleroblasts, there were several types
of cells having different gene expression. Co-expression of birc2 and boka in the cells along the
ridge also suggested that anti-apoptotic mechanism worked to maintain the ridges. birc2 positive
cells along the tube were associated with RANK-positive cells suggesting that these cells were
induced apoptosis by osteoclast-phenotype cells so that apoptosis seems play an important role to
maintain the tube shape.
Disclosures: S.N. Do, None; H. Katsuyama, None; S. Abe, None; K. Saijoh , None; L. Li,
None; A. Ishizaki, None; H. Tanii, None.
Poster
233. Nicotinic Aacetylcholine Receptors: Regulation and Function II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 233.1/C3
Topic: B.01.d. Monoamines: Intracellular signaling cascades
Support: Philip Morris USA (MJE)
SBU MD with Recognition Program (BB and JS)
NIH Grant NS37685 (AST)
Title: Nicotine stimulates signal transduction pathways in pheochromocytoma cells