[Abstract Title]. - Society for Neuroscience

evoked cholinergic signal amplitudes were significantly smaller (WT: 3.05±0.16 κM; β2 KO:
1.78±0.64 κM). The decay rate (t50) of nicotine-evoked signals did not differ between the strains
(WT: 14.47±2.42s; β2 KO: 14.67±0.97s). ABT-089 did not elicit cholinergic transients in β2 KO
mice. In α7 KO mice, the amplitudes of nicotine and ABT-089 evoked cholinergic transients
were identical to those recorded in WT mice. Nicotine-evoked cholinergic signals decayed faster
in α7 KO mice (10.61±1.09s). These results are consistent with the hypothesis that the effects of
ABT-089 are selectively mediated via β2-, but not α7-, containing nAChRs. Furthermore, α7containing
nAChRs contribute to the regulation of cholinergic signal decay rate following
nicotine administration. Ongoing experiments will determine if the amplitudes of glutamatergic
signals in WT and KO mice predict the amplitudes of cholinergic signals. These experiments
will test a neuropharmacological model suggesting that α4β2 nAChRs on glutamatergic
(thalamic) afferents increase prefrontal glutamate release which in turn evokes cholinergic
signals via NMDA and AMPA heteroreceptors located on cholinergic terminals. The continuing
and slowly decaying release of acetylcholine evoked by nicotine is mediated in part via α7
nAChRs and is predicted to be associated with less efficacious cognitive enhancement than the
“sharp” signals evoked by α4β2-selective nAChR agonists.
Disclosures: J. Ji, None; V. Parikh, None; M.W. Decker, Abbott Laboratories, A.
Employment (full or part-time); Abbott Laboratories, E. Ownership Interest (stock, stock
options, patent or other intellectual property); M. Sarter, Abbott Laboratories., B. Research
Grant (principal investigator, collaborator or consultant and pending grants as well as grants
already received); Abbott Laboratories., C. Other Research Support (receipt of drugs, supplies,
equipment or other in-kind support); Abbott Laboratories., D. Speakers Bureau/Honoraria
(speakers bureau, symposia, and expert witness).
Poster
290. Animal Cognition and Behavior: Learning and Memory: Pharmacology I
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 290.20/RR71
Topic: F.02.j. Learning and memory: Pharmacology
Support: CONACYT Scholarship 18533
Title: Memory formation and camp production: 5-ht1a and 5-ht7 receptors
Authors: *G. P. GARCIA, A. MENESES;
Dept Pharmacobiology, CINVESTAV, México, DF, Mexico