[Abstract Title]. - Society for Neuroscience

incubation buffer (10 mM Na-Phosphate buffer, pH 7.4) and specific binding was assessed in
mailers containing 20 pM 125 I-RTI-55 ([ 125 I]-(-)-2β-Carbomethoxy-3β-(4-iodophenyl)tropane]
specific activity of 2200 Ci/mmol), and 200 nM GBR12935 (1-(2-Diphenylmethoxyethyl)-4-(3phenylpropyl)piperazine
dihydrochloride) for 120 min. Non-specific binding was measured in
the presence of 1 mM of paroxetine and 200 nM GBR12935. The brain slices were washed twice
for 20 minutes in a cold buffer, and then quickly immersed in ice cold water, and left over night
to air dry. The sections, along with I-125 standards, were exposed to the Fuji fluorescence plates
for several hours and calibrated as nCi/mg-tissue. The plates were scanned and images were
quantified by a calibration curve obtained from standards in a MCID imaging system. The
binding was quantified in twenty five brain regions. Regional readings were transformed to
bindings (fmol/mg) to assess any possible influence of fluoxetine treatment on the SERT
densities in the FSL and FRL groups.
Four groups ANOVA showed highly significant brain region*group interaction; F(72,480)=12.5;
p