[Abstract Title]. - Society for Neuroscience

fitted with indwelling catheters that allow undisturbed blood collection. SS (n=5) monkeys did
not differ from HSR (n=6) or MSR (n=5) monkeys in cortisol secretion over a 24-hour period
(F13,2=2.135, p=0.158), in response to an acute psychological stressor (F13,2=0.353, p=0709), or
in response to dexamethasone negative feedback (F13,2=0.463, p=0.639). In another group of
animals categorized for degree of sensitivity of the reproductive axis to stress, we collected brain
tissue in the early follicular phase of a control, nonstressed menstrual cycle.
Immunocytochemical staining of CRH fibers was examined in SS (n=3) and HSR (n=4)
monkeys. The immunostained area (µm) was significantly greater in both the dorsal raphe
nucleus (HSR: 64,083±5,670; SS: 116,785±12,734; p=0.009) and median raphe nucleus (HSR:
57,842±7,347; SS: 89,225±8,632; p=0.039) of SS animals. We conclude that increased
sensitivity to stress-induced reproductive dysfunction is not associated with elevated adrenal axis
activity, but that CRH neurons in the caudal PVN might be contributing in a non-neuroendocrine
manner to suppress serotonergic function in the raphe nucleus of animals that show increased
sensitivity to stress-induced reproductive dysfunction.
Disclosures: S.M. Herod, None; M.L. Centeno, None; C.L. Bethea, None; J.L. Cameron,
None.
Poster
283. Stress-Regulated Pathways II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 283.4/OO34
Topic: E.06.f. Stress modulated pathways
Support: NWO Grant 864.05.008
EURON MEST-CT-2005-020589
Title: Exon-specific regulation of BDNF transcripts in the urocortin1 neurons of the nonpreganglionic
Edinger-Westphal nucleus
Authors: *B. GASZNER 1,2 , N. DERKS 2 , M. MAREIKE 2 , E. W. ROUBOS 2 , T. KOZICZ 2 ;
1 Anat., Univ. of Pecs, Pecs, Hungary; 2 Donders Ctr. for Neurosci., Dept. of Cell. Animal
Physiol., Nijmegen, Netherlands
Abstract: Brain-derived neurotrophic factor (BDNF), a growth factor regulated through the
expression of nine exons that each have their specific promoter, is expressed mainly in the limbic
forebrain and hippocampus, where it is involved in neuronal growth and differentiation. BDNF
was implicated in the pathogenesis of stress-related mood disorders such as depression, which